LOCUS       sequence-160933-        1138 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of the human Huntingtin Exon1 protein containing 15Q in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
  TITLE     An Intein-based Strategy for the Production of Tag-free Huntingtin 
            Exon 1 Proteins Enables New Insights into the Polyglutamine 
            Dependence of Httex1 Aggregation and Fibril Formation.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 
            10.1074/jbc.M116.713982. Epub 2016 Mar 21.
  PUBMED    27002149
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.713982"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "12074-86"
COMMENT     Sequence Label: pTWIN1-His6-Ssp-Httex1-15Q
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(846..864)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      860..907
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        967..1071
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttcccctcta gaantntttt gtttacttta agaaggagat atacatatgc atcatcatca
       61 tcaccatagc ggtgcaatta gcggtgatag cctgattagc ctggcaagca ccggtaaacg
      121 tgttagcatt aaagatctgc tggatgagaa agattttgag atttgggcca ttaacgagca
      181 gaccatgaaa ctggaaagcg caaaagttag ccgtgttttt tgcaccggta aaaaactggt
      241 gtatatcctg aaaacccgtc tgggtcgtac cattaaagca accgcaaatc atcgttttct
      301 gaccattgat ggttggaaac gtctggatga actgagcctg aaagaacata ttgcactgcc
      361 tcgtaaactg gaaagtagca gcctgcaact gagtccggaa attgaaaaac tgagccagag
      421 cgatatctat tgggatagca ttgttagcat taccgaaacc ggtgttgaag aagtttttga
      481 tctgaccgtt ccgggtccgc acaattttgt tgcaaatgat attattgtgc acaacgccac
      541 cctggaaaaa ctgatgaaag catttgaaag cctgaaaagc tttcagcagc agcaacaaca
      601 gcagcaacag caacaacaac aacagcagcc tccgcctccg cctcctccac cacctcctcc
      661 acagctgcct cagcctccac cgcaggcaca gcctctgctg ccacagcctc agccacctcc
      721 accgccaccg cctccacctc cgggtccagc agttgcagaa gaaccgctgc atcgtccgta
      781 ataactgcag gaaggggatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      841 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      901 ttttttgctg aaaggaggaa ctatatccgg ataactacgt caggtggcac ttttcgggga
      961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag twtgagtatt
     1081 caacattnnc gtgtcsncct tattcccttt tttgcggcat tttgccttcc tnnttttt
//
