LOCUS       sequence-160934-        1062 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of the human Huntingtin Exon1 protein containing 23Q in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
  TITLE     An Intein-based Strategy for the Production of Tag-free Huntingtin 
            Exon 1 Proteins Enables New Insights into the Polyglutamine 
            Dependence of Httex1 Aggregation and Fibril Formation.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 
            10.1074/jbc.M116.713982. Epub 2016 Mar 21.
  PUBMED    27002149
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.713982"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "12074-86"
COMMENT     Sequence Label: pTWIN1-His6-Ssp-Httex1-23Q
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(856..874)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      870..917
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg ttnactttaa gaaggagata tacatatgca tcatcatcat caccatagcg
       61 gtgcaattag cggtgatagc ctgattagcc tggcaagcac cggtaaacgt gttagcatta
      121 aagatctgct ggatgagaaa gattttgaga tttgggccat taacgagcag accatgaaac
      181 tggaaagcgc aaaagttagc cgtgtttttt gcaccggtaa aaaactggtg tatatcctga
      241 aaacccgtct gggtcgtacc attaaagcaa ccgcaaatca tcgttttctg accattgatg
      301 gttggaaacg tctggatgaa ctgagcctga aagaacatat tgcactgcct cgtaaactgg
      361 aaagtagcag cctgcaactg agtccggaaa ttgaaaaact gagccagagc gatatctatt
      421 gggatagcat tgttagcatt accgaaaccg gtgttgaaga agtttttgat ctgaccgttc
      481 cgggtccgca caattttgtt gcaaatgata ttatcgtgca taatgccacc ctggaaaaac
      541 tgatgaaagc atttgaaagc ctgaaaagct ttcagcaaca acagcagcaa cagcaacagc
      601 agcagcagca gcaacaacag cagcagcaac agcaacaaca accgcctccg cctccgccac
      661 ctcctcctcc accacagctg cctcagcctc cgcctcaggc acagcctctg ctgccacagc
      721 ctcagccacc tccaccgcct ccacctccac ctccgggtcc ggcagttgca gaagaaccgc
      781 tgcatcgtcc gtaactgcag gaaggggatc cggctgctaa caaagcccga aaggaagctg
      841 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      901 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg ataactacgt caggtggcac
      961 ttttcgggga aatgkgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1021 gtatccgstc atgaramaat aaccctgatn aatgcttcaa ta
//
