LOCUS       sequence-160936-        1073 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of the human Huntingtin Exon1 protein containing 37Q in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Vieweg S, Ansaloni A, Wang ZM, Warner JB, Lashuel HA
  TITLE     An Intein-based Strategy for the Production of Tag-free Huntingtin 
            Exon 1 Proteins Enables New Insights into the Polyglutamine 
            Dependence of Httex1 Aggregation and Fibril Formation.
  JOURNAL   J Biol Chem. 2016 Jun 3;291(23):12074-86. doi: 
            10.1074/jbc.M116.713982. Epub 2016 Mar 21.
  PUBMED    27002149
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M116.713982"; journalName: "J Biol Chem"; date: 
            "2016-06-3- 3"; volume: "291"; issue: "23"; pages: "12074-86"
COMMENT     Sequence Label: pTWIN1-His6-Ssp-Httex1-37Q
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(895..913)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      909..956
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tnantttgtt tactttagaa ggagatatac atatgcatca tcatcctcnn catagcggng
       61 cawttagcgg tgatagcctg attagcctgg caagcaccgg taaacgtgnt agcattaaag
      121 atctgctgga tgagaaagat tttgagattt gggccattaa cgagcagacc atgaaactgg
      181 aaagcgcaaa agttagccgt gttttttgca ccggtaaaaa actggtgtat atcctgaaaa
      241 cccgtctggg tcgtaccatt aaagcaaccg caaatcatcg ttttctgacc attgatggtt
      301 ggaaacgtct ggatgaactg agcctgaaag aacatattgc actgcctcgt aaactggaaa
      361 gtagcagcct gcaactgagt ccggaaattg aaaaactgag ccagagcgat atctattggg
      421 atagcattgt tagcattacc gaaaccggtg ttgaagaagt ttttgatctg accgttccgg
      481 gtccgcacaa ttttgttgca aatgatatta ttgtgcacaa cgccaccctg gaaaaactga
      541 tgaaagcatt tgaaagcctg aaaagctttc agcagcagca acaacagcag caacagcaac
      601 agcagcagca gcagcaacaa cagcagcagc aacagcaaca acaacaacaa cagcagcaac
      661 aacagcaaca gcagcagcaa ccgcctccgc ctccgccacc tcctcctcca ccacagctgc
      721 ctcagcctcc gcctcaggca cagcctctgc tgccacagcc tcagccacct ccaccgcctc
      781 cacctccacc tccgggtccg gcagttgcag aagaaccgct gcatcgtccg taactgcagg
      841 aaggggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      901 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      961 aaggaggaac tatatccgga taactacgtc aggnggcact tttcggggaa atgtgcgcgg
     1021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgntca tga
//
