LOCUS       sequence-160960-         911 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            4xMyc (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB418
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(157..281)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(300..329)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(336..365)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(381..410)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(417..446)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(482..827)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(519..539)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg cccctcgagg ggccgccata gtgactggat atgttgtgtt
      181 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      241 tcattttacg tttctcgttc agcttttttg tacaaacttg tgataacagc gctaccgttc
      301 aagtcttcct cggagattag cttttgttca ccgttcaaat cttcttcaga aatcaacttt
      361 tgttcaccgt cgagtccgtt caagtcttct tctgagatta atttttgttc accgttcaag
      421 tcttcctcgg agattagctt ttgttcaccg ttaattaacc cgctcattct agagtccccc
      481 gtgttctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
      541 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
      601 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
      661 gaccactgtc ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt
      721 tgtaggagcc accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat
      781 ggaatccgag gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt
      841 cttctgagac tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt
      901 tgacgaagat t
//
