LOCUS       sequence-161116-       10413 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR 
            gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-m...
FEATURES             Location/Qualifiers
     source          1..10413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..75)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        247..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             844..1024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1071..1196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1695..1928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2113..2157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2455..2572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2825..2858)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2862..2882
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        3176..3479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3480..3683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4293..4442
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4449..4469
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4899..5270
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             5289..5858
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_feature    join(5876..6449,6450..6452,6453..6461)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6043..6060)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6270..6289
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6486..7193
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6628..6646)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6896..6915
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(7213..7246)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7302..7890
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7355..7375)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7773..7784)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7893..7909)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7894..7910)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8419..8599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8661..9249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8741..8760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9420..10211,10212..10280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10043..10062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10281..10385)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggtacgt tctctatcac tgatagttta agagctatgc tggaaacagc atagcaagtt
     3001 taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct
     3061 cgagtactag gatccattag gcggccgcgt ggataaccgt attaccgcca tgcattagtt
     3121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     3181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     3241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     3301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     3361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     3421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     3601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     3661 gggaggtcta tataagcaga gctggtttag tgaaccgtgc tagctttgca tgcggagcta
     3721 ccatgggtgg gggcgcgcca actcaagacg aattcaccca actctcccaa tcaatcgccg
     3781 agttccacac gtaccaactc ggtaacggcc gttgctcatc tctcctagct cagcgaatcc
     3841 acgcgccgcc ggaaacagta tggtccgtgg tgagacgttt cgataggcca cagatttaca
     3901 aacacttcat caaaagctgt aacgtgagtg aagatttcga gatgcgagtg ggatgcacgc
     3961 gcgacgtgaa cgtgataagt ggattaccgg cgaatacgtc tcgagagaga ttagatctgt
     4021 tggacgatga tcggagagtg actgggttta gtataaccgg tggtgaacat aggctgagga
     4081 attataaatc ggttacgacg gttcatagat ttgagaaaga agaagaagaa gaaaggatct
     4141 ggaccgttgt tttggaatct tatgttgttg atgtaccgga aggtaattcg gaggaagata
     4201 cgagattgtt tgctgatacg gttattagat tgaatcttca gaaacttgct tcgatcactg
     4261 aagctatgaa cgtgatcact gcaggtacta gtgatgcttt agacgatttt gacttagata
     4321 tgcttggttc agacgcgtta gacgacttcg acctagacat gttaggctca gatgcattgg
     4381 acgacttcga tttagatatg ttgggctccg atgccctaga tgactttgat ctagatatgc
     4441 taggtagtcc caaaaagaag aggaaagtgg gatcccagta tctgcccgac acagatgata
     4501 gacaccgaat cgaagagaaa cgcaagcgaa cgtatgaaac cttcaaatcg atcatgaaga
     4561 aatcgccctt ctcgggtccg accgatccca ggcccccacc gagaaggatt gcggtcccgt
     4621 cccgctcgtc ggccagcgtg ccgaagcctg cgccgcagcc ctaccccttc acgtcgagcc
     4681 tgagcacaat caattatgac gagttcccga cgatggtgtt cccctcggga caaatctcac
     4741 aagcctcggc gctcgcacca gcgcctcccc aagtccttcc gcaagcgcct gccccagcgc
     4801 ctgcaccggc aatggtgtcc gccctcgcac aggcccctgc gcccgtcccc gtgctcgcgc
     4861 ctggaccgcc ccaggcggtc gctccaccgg ctccgaagcc gacgcaggcc ggagagggaa
     4921 cactctccga agcacttctt caactccagt ttgatgacga ggatcttgga gcactccttg
     4981 gaaactcgac agaccctgcg gtgtttaccg acctcgcgtc agtagataac tccgaatttc
     5041 agcagctttt gaaccagggt atcccggtcg cgccacatac aacggagccc atgttgatgg
     5101 aataccccga agcaatcacg agacttgtga cgggagcgca gcggcctccc gatcccgcac
     5161 ccgcaccttt gggggcacct ggcctcccta acggactttt gagcggcgac gaggatttct
     5221 cctccatcgc cgatatggat ttctcagcct tgctgtcaca gatttccagc ggctctggca
     5281 gcggcagccg ggattccagg gaagggatgt ttttgccgaa gcctgaggcc ggctccgcta
     5341 ttagtgacgt gtttgagggc cgcgaggtgt gccagccaaa acgaatccgg ccatttcatc
     5401 ctccaggaag tccatgggcc aaccgcccac tccccgccag cctcgcacca acaccaaccg
     5461 gtccagtaca tgagccagtc gggtcactga ccccggcacc agtccctcag ccactggatc
     5521 cagcgcccgc agtgactccc gaggccagtc acctgttgga ggatcccgat gaagagacga
     5581 gccaggctgt caaagccctt cgggagatgg ccgatactgt gattccccag aaggaagagg
     5641 ctgcaatctg tggccaaatg gacctttccc atccgccccc aaggggccat ctggatgagc
     5701 tgacaaccac acttgagtcc atgaccgagg atctgaacct ggactcaccc ctgaccccgg
     5761 aattgaacga gattctggat accttcctga acgacgagtg cctcttgcat gccatgcata
     5821 tcagcacagg actgtccatc ttcgacacat ctctgttttg agtttaaaca attcgcccct
     5881 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5941 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     6001 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6061 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6121 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6181 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6241 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6301 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6361 gctttacgtg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     6421 ggttttcctt tgaaaaacac gatgataata tggccacaac cggatccgga gctaccatgc
     6481 ccggggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6541 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6601 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6661 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     6721 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     6781 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     6841 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     6901 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     6961 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7021 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7081 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7141 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagaattcg
     7201 tcgagggacc taataacttc gtatagcata cattatacga agttatacat gtttaagggt
     7261 tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca
     7321 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7381 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7441 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7501 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7561 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7621 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7681 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7741 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7801 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7861 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
     7921 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     7981 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     8041 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8101 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     8161 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     8221 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     8281 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     8341 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     8401 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8461 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8521 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8581 gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8821 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9061 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9181 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9301 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9361 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9661 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10021 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10261 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10321 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10381 ccgcgcacat ttccccgaaa agtgccacct gac
//
