LOCUS       sequence-161127-         914 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Vector targeting the LacZ gene to the GAPDH locus of human cells. 
            LacZ is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-LacZ-IRESMuro
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     155..177
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(210..230)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(240..293)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gcttctggtg ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg
       61 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
      121 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      181 ggatcatcgc gagccatgcg ggggtcttct acctttctct tcttttttgg agccatcgcc
      241 gggccaggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctcctccg
      301 gacccgccgc cacgcgccat tccctccttg gaggccatgt gggccatgag gtccaccacc
      361 ctgttgctgt agccaaattc gttgtcatac ctagaagatg aaaagagttg tcagggccct
      421 ttttctgagc cagccaccag agggcgccag accctgcact ttttaagagc cagtctctgg
      481 ccccagccac ataccaggaa atgagcttga caaagtggtc gttgagggca atgccagccc
      541 cagcgtcaaa ggtggaggag tgggtgtcgc tgttgaagtc agaggagacc acctggtgct
      601 cagtgtagcc caggatgccc ttgagggggc cctccgacgc ctgcttcacc accttcttga
      661 tgtcatcata tttggcaggt ttttctagac ggcaggtcag gtccaccact gacacgttgg
      721 cagtggggac acggaaggcc atgccagtga gcttcccgtt cagctcaggg atgaccttgc
      781 ccacagcctt ggcagcgcca gtagaggcag ggatgatgtt ctggagagcc ccgcggccat
      841 cacgccacag tttcccggag gggccatcca cagtcttctg ggtggcagtg atggcatgga
      901 ctgtggtctg caaa
//
