LOCUS       sequence-161153-         864 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Destination vector for integration at yeast Trp locus with 
            N-terminal TPL repression domain (RD) for auxin response assays .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Pierre-Jerome E, Jang SS, Havens KA, Nemhauser JL, Klavins E
  TITLE     Recapitulation of the forward nuclear auxin response pathway in 
            yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9407-12. doi: 
            10.1073/pnas.1324147111. Epub 2014 Jun 16.
  PUBMED    24979769
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1324147111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9407-12"
COMMENT     Sequence Label: pGP4G-TPLRD1-ccdB
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    418..542
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(567..572,573..590,591..597)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(717..736)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccagaact tagtttcgac
       61 ggattctaga actagtggat cccccgggac aaaatgtctt ctcttagtag agagctcgtt
      121 ttcttgatct tacagtttct cgatgaagag aagtttaaag agactgttca caagcttgaa
      181 caagaatctg ggtttttctt caatatgaag tattttgagg atgaggttca caatggaaat
      241 tgggatgagg ttgagaagta tctctctggt ttcactaaag tagatgataa tagatactct
      301 atgaagatat tcttcgagat aagaaagcag aagtatctcg aggctttgga taagcatgat
      361 cgtcccaagg ctgtggatat actagtgaag gatggtggac caggtggtgg acatcaaaca
      421 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      481 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      541 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      601 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      661 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      721 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      781 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      841 cccgcctgat gaatgctcat ccgg
//
