LOCUS       sequence-161319-         810 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  red fluorophore without stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-mKate2 no stop
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(113..808)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tatctgtgcc
      121 ccagtttgct agggaggtcg cagtatctgg ccacagccac ctcgtgctgc tcgacgtagg
      181 tctctttgtc ggcctccttg attctttcca gtcttctgtc cacatagtag acgccgggca
      241 tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt cttcaagttg cagatcaggt
      301 ggcccccgcc cacgagcttc agggccatgt cggctctgcc ttccaggccg ccgtcagcgg
      361 ggtacagggt ctcggtggag gcctcccagc cgagtgtttt cttctgcatc acagggccgt
      421 tggatgggaa gttcacccct ctgatcttga cgttgtagat gaggcagccg tcctggaggc
      481 tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact ctctcccatg
      541 tgaagccctc ggggaaggac tgcttaaaga agtcggggat gccctgggtg tggttgatga
      601 aggttttgct gccgtacatg aagctggtag ccaggatgtc gaaggcgaag gggagagggc
      661 cgccctcgac cgccttgatt ctcatggtct gggtgccctc gtagggcttg ccttcgccct
      721 cggatgtgca cttgaagtgg tggttgttca cggtgccctc catgtacagc ttcatgtgca
      781 tgttctcctt aatcagctcg ctcaccatgg
//
