LOCUS       sequence-161534-         777 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cre dependent expression of GCaMP6f Ca sensor and physically 
            separate nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(132..165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(266..289)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(464..483)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     733..751
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 catttcaggt gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata
       61 ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat
      121 gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc gccgtcgact
      181 tacttgtaca gctcgtccat gccgtacagg aacaggtggt ggcggccctc ggagcgctcg
      241 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg
      301 tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc
      361 aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg
      421 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
      481 gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc
      541 tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt catcacgcgc
      601 tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc ggcggggtgc
      661 ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca ggcgaagggc
      721 agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcgg
//
