LOCUS       sequence-161731-       10958 bp    DNA     circular SYN 14-APR-2025
DEFINITION  mouse PM20D1, C-term flag and his tags, in mammalian retroviral 
            expression plasmid. Amp/Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Long JZ, Svensson KJ, Bateman LA, Lin H, Kamenecka T, Lokurkar IA, 
            Lou J, Rao RR, Chang MR, Jedrychowski MP, Paulo JA, Gygi SP, Griffin
            PR, Nomura DK, Spiegelman BM
  TITLE     The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers
            of Mitochondria.
  JOURNAL   Cell. 2016 Jul 14;166(2):424-35. doi: 10.1016/j.cell.2016.05.071. 
            Epub 2016 Jun 30.
  PUBMED    27374330
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.071"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "424-35"
COMMENT     Sequence Label: pCLHCX-mPM20D1-flag
FEATURES             Location/Qualifiers
     source          1..10958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             589..764
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    827..1026
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1227..1643
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1573..1595
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1611..1627
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1699..2718
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1699..2718
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     enhancer        3139..3518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3519..3722
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3719..3743
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5364..5387
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5409..5426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5436..6024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5489..5509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5907..5918)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6073..6666
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     intron          7013..7078
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7207..7227
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7499..7620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7536..7555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7590..7609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8664..8681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8835..9423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8915..8934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9892..10268)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     10215..10234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10453..10557)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10625..10643
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     10803..10822
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatgtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca ataaaagagc
      601 ccacaacccc tcactcggcg cgccagtctt ccgatagact gcgtcgcccg ggtacccgta
      661 ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg
      721 gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg ctcgtccggg
      781 atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
      841 cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt
      901 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      961 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     1021 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     1081 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     1141 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     1201 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1261 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1321 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1381 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1441 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1501 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1561 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1621 ccctcactcc ttctctaggc gccggaattc cgatctgata gcttgccaca acccgtacca
     1681 aagatggata gatccggaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     1741 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     1801 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     1861 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     1921 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     1981 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     2041 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     2101 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     2161 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     2221 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     2281 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     2341 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     2401 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     2461 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     2521 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     2581 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     2641 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     2701 ccgagggcaa aggaatagag tagatgccga ccgaacaaga gctgatttcg agaacgcctc
     2761 agccagcaac tcgcgcgagc ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg
     2821 gcacagttct cgtccacagt tcgctaagct cgctcggctg ggtcgcggga gggccggtcg
     2881 cagtgattca ggcccttctg gattgtgttg gtccccaggg cacgattgtc atgcccacgc
     2941 actcgggtga tctgactgat cccgcagatt ggagatcgcc gcccgtgcct gccgattggg
     3001 tgcagatccg gccattagcc atattattca ttggttatat agcataaatc aatattggct
     3061 attggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
     3121 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3361 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3541 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     3601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     3661 ccgccccatt gacgcaaatg ggcggtaggc atgtacggtg ggaggtctat ataagcagag
     3721 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     3781 gaagacaccg ggaccgatcc agcctccgcg gccccaagct tatggctgag ctacttgcta
     3841 gcttgcccgc ctgggcagct gtgctccttc tctttttcgc tacggtctcc ggatccactg
     3901 gccctagaag cagggaaaat cggggggcgt cccggatccc ttcccagttc agcgaggagg
     3961 agcgtgtcgc tataaaagag gcgctgaaag gtgccatcca gattcccaca gtgtctttca
     4021 gccacgagga atccaacacc acagcccttg ctgagtttgg agaatatatc cgcaaagcct
     4081 tccctacagt gttccacagc agccttgtcc aacatgaagt cgtggcaaag tatagccacc
     4141 tgttcaccat ccaaggctca gaccccagtt tgcagcccta catgctgatg gctcacattg
     4201 atgtggttcc tgccccggaa gaaggatggg aggtgccccc gttctcaggc ctggaacgca
     4261 atggcttcat ctatggccgg ggtgcgctgg acaacaaaaa ctctgtgatg gcgatcctgc
     4321 atgctttgga gctcctgttg atcagaaact acagccccaa aagatctttc ttcattgctt
     4381 tgggccatga tgaggaggtg tccggggaaa agggggctca gaagatctca gcactcttac
     4441 aggcaagggg tgtccagcta gccttccttg tggatgaagg gagctttatc ttggaaggct
     4501 tcattccaaa cctcgagaag ccagttgcca tgatttcagt cactgagaag ggtgcccttg
     4561 acctcatgct gcaagtaaac atgactccag gccactcttc agctccccca aaggagacaa
     4621 gcattggcat tctttctgcc gctgtcagcc gactggagca gacaccaatg ccgaatatgt
     4681 ttggaggagg gccattgaag aagacaatga agctactggc aaatgagttt tccttcccta
     4741 tcaatatagt cttgagaaac ctgtggctat ttcatcccat tgtgagcagg ataatggaga
     4801 ggaaccccat aacaaatgcg ctggtccgaa ctaccacagc cctcaccatg ttcaatgcag
     4861 gaatcaaggt gaatgtcatc cctccattgg ctcaggctac aatcaactgc cgaattcacc
     4921 cttcgcagac agtacatgag gtcctagaac ttgtcaagaa caccgtggct gatgacagag
     4981 tccagctgca tgtgttgaga tcctttgaac ccctgcccat cagcccctct gatgaccagg
     5041 ccatgggcta ccagctgctt caagagacca tacgatctgt cttcccggaa gtcgacatcg
     5101 tcgtccccgg tatttgtatt gccaatacgg acacccgaca ctatgccaac atcaccaatg
     5161 gcatgtaccg gttcaacccc cttcccctga accctcagga cttcagtggt gtccatggaa
     5221 tcaatgagaa agtttccgtt cagaactacc agaaccaggt gaagttcatc tttgagttca
     5281 tccaaaatgc cgacacttac aaagagccag ttcctcatct gcatgaacta acgcgtccca
     5341 gatctcccac cggtcccgga tccgactaca aggacgatga cgacaaggag ctgaagaccg
     5401 cggccgcgca tcatcaccat caccattaac tcgagaatca acctctggat tacaaaattt
     5461 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5521 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5581 ataaatcctg gttgctgtcc ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5641 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5701 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5761 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5821 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5881 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5941 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6001 tctccctttg ggccgcctcc ccgcctgatc gataaaataa aagattttat ttagtctcca
     6061 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     6121 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     6181 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     6241 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     6301 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     6361 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     6421 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     6481 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     6541 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     6601 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     6661 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
     6721 cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6781 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6841 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     6901 cgatagcgga gtgtatacct agctaggtag ctagaggatc tttgtgaagg aaccttactt
     6961 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     7021 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     7081 tccaacctat ggaactgatg aatgggagca gtggtccaat gcctttaatg aggaaaacct
     7141 gttttgctca gaagaaatgc ctctagtgat gatgaggcta ctgctgactc tcaacattct
     7201 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     7261 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     7321 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt
     7381 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7441 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7621 tcatgtctga tcccgccatg gtatcaacgc catatttcta tttacagtag ggacctcttc
     7681 gttgtgtagg accgctgtat tcctagggaa atagtagagg caccttgaac tgtctgcatc
     7741 agccatatag cccccgctgt tcgacttaca aacacaggca cagtactgac aaacccatac
     7801 acctcctctg aaatacccat agttgctagg gctgtctccg aactcattac accctccaaa
     7861 gtcagagctg taatttcgcc atcaagggca gcgagggctt ctccagataa aatagcttct
     7921 gccgagagtc ccgtaagggt agacacttca gctaatccct cgatgaggtc tactagaata
     7981 gtcagtgcgg ctcccatttt gaaaattcac ttacttgatc agcttcagaa gatggcggag
     8041 ggcctccaac acagtaattt tcctcccgac tcttaaaata gaaaatgtca agtcagttaa
     8101 gcaggaagtg gactaactga cgcagctggc cgtgcgacat cctcttttaa ttagttgcta
     8161 ggcaacgccc tccagagggc gtgtggtttt gcaagaggaa gcaaaagcct ctccacccag
     8221 gcctagaatg tttccaccca atcattacta tgacaacagc tgtttttttt agtattaagc
     8281 agaggccggg gacccctggc ccgcttactc tggagaaaaa aaacattgta gaggcttcca
     8341 gaggcaactt gtcaaaacag gactgcttct atttctgtca cactgtctgg ccctgtcaca
     8401 aggtccagca cctccatacc ccctttaata agcagtttgg gaacgggtgc gggtcttact
     8461 ccgcccatcc gcccctaact ccgcccagtt ccgcccattc tccgccccat gctgactaat
     8521 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8581 aggaggcttt tttggaggct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8641 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9181 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9241 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9301 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9361 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9421 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9481 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9541 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9601 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9661 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9721 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9781 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9841 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9901 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9961 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10021 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10081 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10141 ggtgaactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10561 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10621 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10681 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10741 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10801 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10861 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10921 ggaagggcga tcggtgcggg cctcttcgct attacgcc
//
