LOCUS       sequence-161793-         971 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Used to evaluate the expression output of CUP1 promoter with yEGFP 
            used as the reporter gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE
  TITLE     Controlling heterologous gene expression in yeast cell factories on 
            different carbon substrates and across the diauxic shift: a 
            comparison of yeast promoter activities.
  JOURNAL   Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5.
  PUBMED    26112740
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2015-06-26"; volume: "14:91. doi"; pages: " 
            10.1186/s12934-015-0278-5"
COMMENT     Sequence Label: pILGFPI7
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(130..364)
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     protein_bind    564..597
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt
       61 tagaggatcc cattttatgt gatgattgat tgattgattg tacagtttgt ttttcttaat
      121 atctatttcg atgacttcta tatgatattg cactaacaag aagatattat aatgcaattg
      181 atacaagaca aggagttatt tgcttctctt ttatatgatt ctgacaatcc atattgcgtt
      241 ggtagtcttt tttgctggaa cggttcagcg gaaaagacgc atcgctcttt ttgcttctag
      301 aagaaatgcc agcaaaagaa tctcttgaca gtgactgaca gcaaaaatgt ctttttctaa
      361 ctagtaacaa ggctaagata tcagcctgaa ataaagggtg gtgaagtaat aattaaatca
      421 tccgtataaa cctatacaca tatatgagga aaaataatac aaaagtgttt taaatacaga
      481 tacatacatg aacatatgca cgtatagcgc ccaaatgtcg gtaatgggat cggcttagtc
      541 gactttctcg agcaaccctt aatataactt cgtataatgt atgctatacg aagttattag
      601 gtctagagat cccaatacaa cagatcacgt gatctttttg taagatgaag ttgaagtagt
      661 gttgcaccgt gccaatgcag gtggctatta gattaaatat gtgatttgtt ctattaagtt
      721 tcctgtataa ttaatgggga gcgctgattc tcttttggta cgcttcccat ccagcatttc
      781 tgtatctttc accttcaacc ttaggatctc tacccttggc gaaaagtcct ctgccaacaa
      841 tgatgatatc tgatccacca cttacaactt cgtcgacggt tctgtactgc tgacccaatg
      901 catcgccttt gtcgtctaaa cctacacctg gggtcatgat tagccaatca aacccttctt
      961 ctcttcctcc c
//
