LOCUS       sequence-161854-        4239 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing DOK1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DOK1_p.P419S
FEATURES             Location/Qualifiers
     source          1..4239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2118..2217)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2234..2253)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2235..2253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2258..2274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2258..2274)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2705..3496
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3589..4177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4078..4097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggacggag cagtgatgga agggccgctt tttttgcaga gtcagcgctt
      721 tgggaccaag aggtggagga agacctgggc cgtgctctac ccggccagtc cccacggcgt
      781 agcgcggctc gagttctttg accataaggg gtcgagctct gggggtggcc gagggagctc
      841 gcgccgcctg gactgcaaag tgatccgtct ggctgagtgt gtgagtgtgg cccccgtcac
      901 cgtggagacc ccccctgagc ccggcgccac tgccttccgc ctggacactg ctcagcgctc
      961 gcacctgctg gcggccgacg cgccgtccag tgcagcctgg gtgcagacgc tgtgccgaaa
     1021 cgcctttccg aaaggcagct ggactctggc gcctaccgat aacccaccta agctttctgc
     1081 cctggagatg ctggagaact ccttgtacag ccctacctgg gaaggatccc aattctgggt
     1141 aacggtgcag aggactgagg ccgccgagcg ctgtggcctg catggctcct acgtgctgag
     1201 ggtggaggct gaaaggctga ctctcctgac cgtgggggcc cagagtcaga tactggagcc
     1261 actcctgtcc tggccctaca ctctgttgcg tcgctatggc cgggacaagg tcatgttctc
     1321 tttcgaggcc ggccgccgct gcccctcagg ccctggaacc ttcaccttcc agacggcaca
     1381 gggaaatgac atcttccagg cagttgagac tgccatccac cggcagaagg cccagggaaa
     1441 ggccggacag gggcacgatg ttctcagagc tgactcccat gaaggggagg tggcagaggg
     1501 gaagttgcct tccccacctg gcccccaaga gctcctcgac agtcccccag ccctgtatgc
     1561 tgagccctta gactccctgc gcattgctcc atgcccttcc caggactccc tatactcaga
     1621 ccccttggac agcacgtctg ctcaggcagg agagggagta caacggaaga aacctctcta
     1681 ttgggacttg tatgagcatg cgcagcagca gttgctgaag gccaagctga cagaccccaa
     1741 agaggatccc atctatgatg aacctgaggg cctggcccca gtccctcccc agggccttta
     1801 tgatctgcct cgggagccca aggatgcatg gtggtgccaa gctcgggtga aggaggaggg
     1861 ctatgagctc ccctacaacc ctgccactga tgactacgct gtgccacccc ctcggagcac
     1921 aaagtccctc cttgctccca agccccaggg cccagccttc cctgaacctg gtactgcaac
     1981 tggcagtggc atcaaaagcc acaactcagc cctgtacagc caggtccaga agagcggggc
     2041 ctcagggagc tgggactgtg ggctctctag agtagggact gacaagactg gggtcaagtc
     2101 agagggctct acctgattgc caactttctt gtacaaagtt ggcattataa gaaagcattg
     2161 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2221 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2281 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2341 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2401 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2461 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2521 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2581 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2641 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2701 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2761 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2821 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     2881 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2941 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3001 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3061 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3121 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3181 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3241 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3301 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3361 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3421 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3481 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3541 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3721 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4201 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
