LOCUS       sequence-161886-         924 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway donor vector for IAA17 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins 
            E, Nemhauser JL
  TITLE     A synthetic approach reveals extensive tunability of auxin 
            signaling.
  JOURNAL   Plant Physiol. 2012 Sep;160(1):135-42. doi: 10.1104/pp.112.202184. 
            Epub 2012 Jul 27.
  PUBMED    22843664
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.112.202184"; journalName: "Plant Physiol"; date: 
            "2012-09"; volume: "160"; issue: "1"; pages: "135-42"
COMMENT     Sequence Label: pDONR-IAA17_no stop
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             100..786
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     protein_bind    complement(788..887)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(904..923)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(905..923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggcttcaaaa tgatgggcag tgtcgagctg
      121 aatctgaggg agactgagct gtgtcttggt cttcccggtg gagatacagt ggctccggta
      181 accggaaaca agagagggtt ctcagagacg gttgatctga agctaaatct gaataatgag
      241 cctgcaaaca aggaaggatc tacgactcat gacgtcgtga cttttgattc caaggagaag
      301 agtgcttgtc ctaaagatcc agccaaacct ccggccaagg cacaagttgt gggatggcca
      361 ccggtgagat cataccggaa gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg
      421 gaggcggcgg cgttcgtgaa ggtatcaatg gacggagcac cgtacttgag gaaaatagat
      481 ttgaggatgt ataaaagcta cgatgagctt tctaatgctt tgtccaacat gttcagctct
      541 tttaccatgg gcaaacatgg aggagaagaa ggaatgatag acttcatgaa tgagaggaaa
      601 ttgatggatt tggtgaatag ctgggactat gttccctctt atgaagacaa agacggtgat
      661 tggatgctcg tcggcgacgt tccttggcca atgttcgtcg atacatgcaa gcgtttacgt
      721 ctcatgaaag gatcggatgc cattggtctc gctccgaggg cgatggagaa gtgcaagagc
      781 agagctcacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      841 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
      901 atcccctata gtgagtcgta ttac
//
