LOCUS       sequence-162101-         959 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Palm1-12GS-I3-01-myc-Late1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-07
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(144..173)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(917..936)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(918..936)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggtcgttac cgaacagaga acgcagagag gtcagcgggt acagttcttt gtcgatcggc
       61 tcctgttttt gcggtggcgt ggtggtttca acaccagaac ggaaagattc ttccggcggt
      121 gcggtcggtt ccggacgaga ctgcagatct tcttcgctaa tcagtttctg ttcggtgcag
      181 ccacgaatct tctcaacgaa cgccttggcc ttttccgcca cttccaccgg ggtgcctttc
      241 actaacgcgc tgcccacgcc cacggccagc acgcccgctt tgaaccattc gcacacattg
      301 tccagattaa caccgccggt tggcacaaat ttcacattcg gaaacggacc tttcatcgct
      361 ttaacaaact gcggacccac cacctcaccc ggaaacagtt ttaagatggt atggcccagt
      421 ttcatggctt tcaccagttc ggtcggggtc ataacgcctg gcatatagaa cacacccttc
      481 tccttgcaaa attggctaat ttcctcgtcc aggtgcggac tcacaatgaa ttcggcgcca
      541 ctttccaccg ctttacggca ctgctccacg ctggtcacgg tgcccgcacc aataatcgcg
      601 cccatttcct tcagaaagct cagttcctta atcacggtat ccgcatccgg cacggtaaag
      661 gtaatttcaa tcagatgaac gccgcccagg aaaaccgcca gcgctttctt tttcgcttct
      721 tccacactat tggcacgcag cacggcaacg atcttgtgtt tcttgaacag ctcttcgatc
      781 ttgctaccag agtcagagcc ggagccagat ttagaaccgt tcaaattatc cttgcgcttg
      841 ctcttgatac aacccatggt acctccggat cgataattct agaggggccc gtttaaacgc
      901 tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagt
//
