LOCUS       sequence-162170-         842 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Cannon MB, Remington SJ
  TITLE     Re-engineering redox-sensitive green fluorescent protein for 
            improved response rate.
  JOURNAL   Protein Sci. 2006 Jan;15(1):45-57. Epub 2005 Dec 1.
  PUBMED    16322566
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2006-01"; volume: "15(1)"; pages: "45-57. Epub 2005 
            Dec 1"
COMMENT     Sequence Label: pRSETB-roGFP1-R10
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..783)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGDLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLKTCSALSKDPNEKRDHMVLLE
                     RVTAAGITHGMDELYK"
     primer_bind     725..753
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(join(802..816,817..825))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tgttagcagc cggatcaagc ttcgaattcc atggtaccag ctgcagatct cgagctcgga
       61 tccttattat ttgtatagtt catccatgcc atgtgtaatc ccagcagctg ttacgcgctc
      121 aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag agcatgtctt
      181 caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata
      241 atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga agttaacttt
      301 gattccattc ttttgtttgt ctgccatgat gtatacattg tggcagttat agttgtattc
      361 caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta actcgattct
      421 attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc
      481 tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc
      541 atgccgtttc atatgatctg ggtatcttga aaagcattga acaccataac tgaaagtagt
      601 gacaagtgtt ggccatggaa caggtagttt tccagtagtg gaaataaatt taagggtaag
      661 atctccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt gcccattaac
      721 atcaccatct aattcaacaa gaattgggac aactccagtg aaaagttctt ctcctttact
      781 catgaattca gcggggggat ccttatcgtc atcgtcgtac agatcccgac ccatttgctg
      841 tc
//
