LOCUS       sequence-162209-         885 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Pentaprobe for assesing the RNA binding potential of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP
  TITLE     A rapid method for assessing the RNA-binding potential of a protein.
  JOURNAL   Nucleic Acids Res. 2012 Aug;40(14):e105. doi: 10.1093/nar/gks285. 
            Epub 2012 Apr 3.
  PUBMED    22492509
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2012-08"; volume: "40(14):e105. doi"; pages: " 
            10.1093/nar/gks285. Epub 2012 Apr 3"
COMMENT     Sequence Label: Pentaprobe PP5
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(223..242)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(224..242)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(287..490)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(316..336)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        491..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatctccaa
       61 cctcacacca cattacactg gggtgatata atatgatgcc gggcgggggc cgtaacgtag
      121 cactacatga cccgatacgc taggtgggat taggatctgc agaattccag cacactggcg
      181 gccgttacta gtggatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat
      241 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      301 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      361 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      421 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
      481 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
      541 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
      601 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
      661 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
      721 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
      781 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
      841 actattaata actagtcaat aatcaatgtc aacgcgtata tctgg
//
