LOCUS       sequence-162269-       10302 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 10302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dRGA[MCS]
FEATURES             Location/Qualifiers
     source          1..10302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6766..7482
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6796..6824)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7419..7439
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(7627..7645)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7628..7645)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7663..7679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7663..7679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7676..7698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7687..7703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7711..7717,7718..7735,7736..7741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7756..7777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7894..7911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8065..8653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8145..8164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8824..9615,9616..9684))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9447..9466
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9685..9789)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9857..9875
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9913..9935)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10035..10054
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10248..10270
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10262..10279
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10263..10279
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10286..10302,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10286..10302,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cctaaggaca tagtcgctag caagcggccg catttaaatc
       61 cgcggcctag gtcccgggta ccctcagcat acatcttttt aagtcgggca ttctccgatt
      121 ctaatgcctt tagcttggta atcatcggta aatccatacc tccatacctt gatctctata
      181 tagacataat ttggctgtca ctatgctttg atatagtcat tttacagaat ctcctttttt
      241 gttattagaa aattctactc ttcttgtcga ttttgtttta gggaggatta ccttctctta
      301 tgtttttgat cctgtgtttt cacttacttt gactataatt ttttaactaa tatccatact
      361 ccactctctt aacccctgca ctagagccaa gcctctgtat aacatgagag taattattac
      421 gcacccaatc cgcaataaat cttgctggca ttgtaaactt tagtgtagaa gtatcattgc
      481 actccttcac tactgctttt tcaaaccaag ctgtatacat agcttctcct aactcttcaa
      541 ttaatccttg tctgatatga tgccatgctg tatttgcttc gatcgtatct aatcccccac
      601 ttttcgctaa aatacctgca tgtgtaccat cagacacaac atcagatgtt ataattttag
      661 gatatagcat taccttttct tcatctctcc gctttctttc attgcttgta tcctccacct
      721 cttctacctt ctgcacataa taaccgttca ttccatatac tgtttctagc tgctcactta
      781 agatctctat ttgtcgctca cttaaagcta ggttacttgg aattaataca gttataaagc
      841 tgttgttggc acttatctta aactctactt gagttagtat tgaatacgct atggaagtgc
      901 taaatcttcc tgctatcttc ttctttacct gcatttcttt actggtatca aatgaattct
      961 ctatttggct taaatattgc tcatgttcta acatgttctg ctcttcagca cctatattac
     1021 aactgaatct aaatgttgta tgattaacaa gtggtccttg atgtttttcg ctcgcaagca
     1081 ctttcttcat atagcttaag aagataaact tattcttaaa tttcttttca ggatatttga
     1141 tatatagctt caatagtaac tggtttacaa agttagtgct aaattccctg tttgctttga
     1201 agttaagtat atctacatct ttttctgata gtggatagaa atgccttaat tctttatttt
     1261 cagatatatc tttgatggtt cttgcaacaa tctcggggtg tatattagca caaggtagct
     1321 taagactagc tgtttcatgg tattcttcct tagaaacaaa acaaccagct tcagaatcat
     1381 ttaaaaaaga agaattgtta taaaattcag atggaaacga gctttgctcg tttcccaaaa
     1441 actctttttt gaaattttct tcagatttat atatgtttga agaatcattt atagataaat
     1501 tcttaagatt tacatctata taatctatat ttttaataat actattcttt gatacaaagt
     1561 ctttactata taattgacca gaatcagtgc aaggagggtc atattctgtg catttccatt
     1621 ctttagcgat ttctaaggct ttattgttat tatactcaat tactatttgt tgcttttgct
     1681 ctttctgttt tttctgatca ttgttattta tgagagatat attcagaaga gatatttgcc
     1741 ataaagattt atcagccctg ctattataat catcattgtt tctgataact aacttatctc
     1801 gcttaataag attattatct tctaaatcct ttaatgctct agaaatagaa tttctatgta
     1861 atttaagttc tttctgtaat tcttgataag aagtgacaat agagtgcaga ttattagtta
     1921 tagagtgatc atggtcataa gcatgatcat aaaaatcttt gtaatcattc ttattgctgt
     1981 tactggcatc aatataagtc tctgtagtga tcctgttgtc tttgtcctta ctgtccgtgt
     2041 ctctgttacc tttatcatta ttactagata atgaagattt ataaatcttt gtaaaaagat
     2101 agagtgttat agtctttgct gttgcagaaa tgctgcaatt agaatttaat tgctttaaga
     2161 actcataagg tactcttata aacatcttag ttttttcaag atgttgcctt tttccttcta
     2221 tggttggcac aaagatatta tctatagaat ctaacttatc tgacttagag tgacgtgttt
     2281 ttgtattatc gatatcacct agatcaaacc tatcaggttc atatttaaga tcattaaaaa
     2341 ccttatcagg tattgttgta gtaataatat tacgtttatt ctgaccttga tcgttcttat
     2401 ctctagtaat aatgaaatgt cctttagctt ccagggattt ctgaagcaca aatacttcag
     2461 atttagagca agatagtctt tctgcccagc ttttagcagg tagagatata gatctattat
     2521 ggcccttaga gaagttaata caagaaagcc catcagctaa gatgtagtac attttctcaa
     2581 gagtagataa ctctttgctt gataatatat gagataatac gcacccagtt attgggacgt
     2641 atgattgatt ctttgtggta tcatagtact gatgtatacc tacattatca ctgggcgtag
     2701 caagcgggta aaatgattgc attttatgct ccttttatgg agctaagtac ctgaaaccct
     2761 tgaccgctac gtagaaatag tatatattta ttatatgtaa aaatcattct gcccgtgttt
     2821 atttttgcat atcatatcaa tcaaggtgtt gagttttagc tattttgagc acttagcacc
     2881 tgtagtttat tgtttcacta tcgtatctat tttatttaat ttatacttct cgagtttatc
     2941 atttgcagca tctataaacc actttctgcg cgtgataaag ttttcagcac aaattttatc
     3001 aatcttttct ataatatgct ctggaatatc taaagatatc ctttttacgt ttattttctt
     3061 cagcataaag ccattgagat aaatgaatta taatcatagt taaacatatt ttgtacacat
     3121 gtcaataaaa ataattattt cacacataaa aaataattat tttaaaatca gagcacataa
     3181 catgtatatg ttaacatata tgtatgtttt tacaatctaa tataaacata ttgacatatg
     3241 tatgtttata tatttatatt atcgtataaa aaataaataa tgttattatg attttgtctt
     3301 tcttgaatca aaaaggaggt gtaggtaaga ctacactttc aattaatgtt gcaacatgtc
     3361 tatcactaaa aaagcaaaaa gtgcttttaa tagatgcgga tccacaaaat agttctctag
     3421 attgggttgc aactcgtaga aaagaaaatt tattcactgt agttgggctc acaaaaccaa
     3481 taatacataa agaggtatca aaattggtta agaattatga tcatataatt attgatggac
     3541 caccaaggat atatgatgtt gctaagtctg caatagttac tagtgacctg gtagtaatgc
     3601 cagtgcaacc gtcaccttat gatatatggg cagctaatga agtggttagc cttataaaag
     3661 aagtatctca accattagaa ggaataaaaa atataaagtc cgccttttta attaatagga
     3721 aaatatccaa tactgctatt ggtcgagatg tggaacaagc gttagatcat tacggtatgg
     3781 atattttaca aacacatatt tgtcagagag tggcatatgc agaaacagca gctattggtt
     3841 ccacagttat ggaaaagcat aatagagaca atctagccgt tagagaaata acagatttaa
     3901 caaatgaaat tttagaaaaa tataactaat tgaaaaggtc atgacaagaa aaagaattgc
     3961 gctccccaaa aaccggtaca aaaaaatact actcaattta ttgatgcatg ggtgtcgggt
     4021 aatataaaac atgaacaaca ggatcaaaaa cataagagaa cgactatctt tttaccagaa
     4081 gaattgcata agaaattaaa aatcgaggcg gctaggaggg aggtaacaat gactgaaata
     4141 attatagaag ctgtagaaaa aagtatatag acatatatat gcttatatgt cttaacttaa
     4201 tgtttttgtt tattaaagca aggatgctta atgttaagca ggatcaaata ctattcatca
     4261 cgtttcttga tcagacttat aactaaattt tagtgtagta caaatcatat aaacatatag
     4321 gaatatgtat gtttatattt tggtcctacc aatcatttca tctatagcga tttgaaaata
     4381 atctgccagt tttactataa ctttaatact taaagtccta ttatcactat ttgtattaat
     4441 aaaatgagta atggtactac tacctactct gcaatttttt tccaatatat actctgtaat
     4501 gttaagttgc tgtatttttg ttttaagaaa agagaataag ttattattaa tatcatttaa
     4561 agttaaagat acatataatt gatatttttt tgtatttttt ataaattttg tgcgaccaag
     4621 tacttcatct atatcacatt caaagaatgt tgcaattata agaatagtga tgatattagg
     4681 attaagtaaa attgcgttta ggagtttgct aatagtagat cgcgataaag atgttcctaa
     4741 taccaaatct ttttgttgta ttttataagc tttaattttt tgacttaaaa atttttgtat
     4801 ccttagtgca atatccataa ataactcaaa atatcataca aatagtttct gaatgccaaa
     4861 aactattttc ttgacacatt tcttgacaca ccaaaaatat atttaacaaa gtagaagtat
     4921 gaggtagtgg attgagctga taataaagta caaactatct tataaaaaga aaaaccaata
     4981 aacactcaaa ttttggacaa ggagcgtgtt gactggtgat taaagtcacc caaactgcaa
     5041 gattagttca catatcttat gaccctaatg ttacgagttt tgtagatcaa gcgtgcaatg
     5101 ctgacaatgc agatcatgat aaccaatcct catcaaaatt acctaccgct aaaacaatca
     5161 cagtgctaat cagtttttta aaactcagca aatatgacga tgagtggtta agagtaggga
     5221 agcaactact gctactgcaa agacctacag ataagcgaag atgctatagg tcagttttat
     5281 tatgttttaa gtcagtaatc aaaacccatt ctgataatta cgtagcatat tactttagcg
     5341 gtataacatt atgtttgctt ggaagttatg acgaagcaat aaagatgttt gatatgagtc
     5401 ttcgctataa caaagaaaaa agcactgctc ttgtacatac atttatctga ggtatcaagc
     5461 atcamagagg gcatcagact tgctacagga aataataaca gtggtaatag tgattgatgc
     5521 acccatgcag aaatgttttt tgttaaataa cgagagttta acagtgaatg agggtcctag
     5581 acaagctaga gaggaaaatc attaaaaaac ttcaaagata caaaatagat ggggtaggga
     5641 aaattagtgc gactaatcac aaacaacgtt cacccaaagg taaaatattg gcatcagcag
     5701 aacaagaaaa atggggtaga tattttgaac tagggatgta ttggcacgga agagaagagg
     5761 gaaatcataa agaattaata tatttgagaa ggtgcttgca gctaaatccc gagaatgaac
     5821 cagcaaattg ttatgtgggg atgatactgc aaaagcaagg cagatatcat gagagcttag
     5881 cggcttacag aaaagcaata tcattaagac ctgagctaaa ggaattagac tcgaagaatg
     5941 cccgacttaa aaagatgtat gctgagggga tcctctagag tcgacctgca gcaacatact
     6001 tgcttttata ggaaaaataa ttataattta agtttactgt aatttacaag ttcttataat
     6061 ttgtatttag atatgattta aagagagtca agggtgaagt atatttttac cctttaatca
     6121 gtgatataag taagtttttt ggagtgtttt catatggtaa aagattggat tcctatttct
     6181 catgataatt ataaacaagt acaaggacca ttttatcatg gaactaaagc taatttagca
     6241 attggtgatt tactaactac aggatttatt tctcattttg aagatggtcg aattcttaaa
     6301 catatttatt tttcagcttt aatggaacca gcagtttggg gagctgaact tgctatgtca
     6361 ctatctggtc ttgaaggtcg tggttatata tatatagttg aaccaacagg accatttgaa
     6421 gatgatccaa atcttacaaa taaaaaattt cctggtaatc caacacaatc ttatagaact
     6481 tgtgaacctt taagaattgt tggtgttgtt gaagattggg aaggacatcc tgttgaatta
     6541 ataagaggaa tgttagattc attagaagat ttaaaacgtc gtggtttaca tgttattgaa
     6601 gattaaggat cctctagagg atccgcaaca aggccaacac cgctacggaa tgacatataa
     6661 tctatacata aaaatatcat ctatgttatt aattataata gacatattaa aaaagttgcg
     6721 ttataacact ttttaagtga tttaattttg attaaggttt tacatatgag taaaggagaa
     6781 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     6841 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     6901 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttctct
     6961 tatggtgttc aatgcttttc ccgttatccg gatcatatga aacggcatga ctttttcaag
     7021 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac
     7081 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta
     7141 aaaggtattg attttaaaga agatggaaac attctcggac acaaactcga gtacaactat
     7201 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc
     7261 aaaattcgcc acaacattga agatggatcc gttcaactag cagaccatta tcaacaaaat
     7321 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct
     7381 gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact
     7441 gctgctggga ttacacatgg catggatgag ctctacaaat aaggctaata agcaaccgtt
     7501 ctataatcat aaaaaaagcc tatatagttt gactatatag gcttttttgc tttataatgt
     7561 agttttgaga agcgtcattg cgaggatccc cagcttgcat gcctgcaggc atgcaagctt
     7621 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
     7681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9781 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     9841 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     9901 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     9961 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10021 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10081 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10141 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10201 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10261 ttgtaaaacg acggccagtg aattgtaata cgactcacta ta
//
