LOCUS       sequence-162394-        3087 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing CCL2, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CCL2_WT_V5
FEATURES             Location/Qualifiers
     source          1..3087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     603..619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     603..619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    660..759
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1057..1156)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1172..1189)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1172..1188)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1181..1203)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1252..1338
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1430..1457
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1616..1633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1787..2375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1867..1886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2468..3087,1..172))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
       61 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
      121 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta
      181 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
      241 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
      301 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
      361 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg
      421 aacaggctta tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
      481 caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag aggaggcatg
      541 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct
      601 gccaggaaac agctatgacc atgtaatacg actcactata ggggatatca gctggatggc
      661 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
      721 gctttcttat aatgccaact ttgtacaaga aagttgggca agtcttcgga gtttgggttt
      781 gcttgtccag gtggtccatg gaatcctgaa cccacttctg cttggggtca gcacagatct
      841 ccttggccac aatggtcttg aagatcacag cttctttggg acacttgctg ctggtgattc
      901 ttctatagct cgcgagcctc tgcactgaga tcttcctatt ggtgaagtta taacagcagg
      961 tgactggggc attgattgca tctggctgag cgagcccttg gggaatgaag gtggctgcta
     1021 tgagcagcag gcacagaagg gcggcagaga ctttcatgcc aacttttttg tacaaagttg
     1081 gcattataaa aaagcattgc tcatcaattt gttgcaacga acaggtcact atcagtcaaa
     1141 ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact
     1201 gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa
     1261 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1321 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga
     1381 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc
     1441 ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa
     1501 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1561 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1621 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1681 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1741 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1801 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1861 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1921 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1981 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2041 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2101 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2161 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2221 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2281 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2341 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2401 ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc
     2461 tcgagggtta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     2521 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     2581 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     2641 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     2701 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     2761 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     2821 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     2881 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     2941 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     3001 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     3061 tcaaagctcg ccgcgttgtt tcatcaa
//
