LOCUS       sequence-162664-         955 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  To fuse your protein of interest to the C-terminus of BioID2 and use
            in proximity-dependent biotin identification (BioID); a small MCS is
            present downstream of myc tag and BioID2 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux 
            KJ
  TITLE     An improved smaller biotin ligase for BioID proximity labeling.
  JOURNAL   Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
  PUBMED    26912792
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2016 Feb 24. pii: mbc.E15-12-0844."
COMMENT     Sequence Label: mycBioID2-pBABE-puro
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             85..780
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     primer_bind     complement(820..840)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cagtgtggtg gtacgtagga attcggagac ccaagctggc tagccaccat ggaacaaaaa
       61 ctcatctcag aagaggatct cgacttcaag aacctgatct ggctgaagga ggtggacagc
      121 acccaggaga gactgaagga gtggaacgtg agctacggca ccgccctggt ggccgacaga
      181 cagaccaagg gcagaggcgg cctgggcaga aagtggctga gccaggaggg cggcctgtac
      241 ttcagcttcc tgctgaaccc caaggagttc gagaacctgc tgcagctgcc cctggtgctg
      301 ggcctgagcg tgagcgaggc cctggaggag atcaccgaga tccccttcag cctgaagtgg
      361 cccaacgacg tgtacttcca ggagaagaag gtgagcggcg tgctgtgcga gctgagcaag
      421 gacaagctga tcgtgggcat cggcatcaac gtgaaccaga gagagatccc cgaggagatc
      481 aaggacagag ccaccaccct gtacgagatc accggcaagg actgggacag aaaggaggtg
      541 ctgctgaagg tgctgaagag aatcagcgag aacctgaaga agttcaagga gaagagcttc
      601 aaggagttca agggcaagat cgagagcaag atgctgtacc tgggcgagga ggtgaagctg
      661 ctgggcgagg gcaagatcac cggcaagctg gtgggcctga gcgagaaggg cggcgccctg
      721 atcctgaccg aggagggcat caaggagatc ctgagcggcg agttcagcct gagaagaagc
      781 ctcgagtgac ttaagtttaa accgctgagt cgaccctgtg gaatgtgtgt cagttagggt
      841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      901 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaag
//
