LOCUS       sequence-162706-         828 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expression of SRSF2 mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Kim E, Ilagan JO, Liang Y, Daubner GM, Lee SC, Ramakrishnan A, Li Y,
            Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, 
            Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis 
            Y, Allain FH, Halene S, Bradley RK, Abdel-Wahab O
  TITLE     SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific 
            Effects on Exon Recognition.
  JOURNAL   Cancer Cell. 2015 May 11;27(5):617-30. doi: 
            10.1016/j.ccell.2015.04.006.
  PUBMED    25965569
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.04"; journalName: "Cancer Cell"; date: 
            "2015-05-11- 11"; volume: "27"; issue: "5"; pages: "617-30"
COMMENT     Sequence Label: pRRL_SRSF2_P95L_mCherry
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             711..734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             735..788
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gttgaccgaa tcaccgacct ctctccccag ggggatccgc tatgagctac ggccgccccc
       61 ctcccgatgt ggagggtatg acctccctca aggtggacaa cctgacctac cgcacctcgc
      121 ccgacacgct gaggcgcgtc ttcgagaagt acgggcgcgt cggcgacgtg tacatcccgc
      181 gggatcgcta caccaaggag tcccgcggct tcgccttcgt tcgctttcac gacaagcgcg
      241 acgctgagga cgctatggat gccatggacg gggccgtgct ggacggccgc gagctgcggg
      301 tgcaaatggc gcgctacggc cgcctcccgg actcacacca cagccgccgg ggaccgccac
      361 cccgcaggta cgggggcggt ggctacggac gccggagccg cagccctagg cggcgtcgcc
      421 gcagccgatc ccggagtcgg agccgttcca ggtctcgcag ccgatctcgc tacagccgct
      481 cgaagtctcg gtcccgcact cgttctcgat ctcggtcgac ctccaagtcc agatccgcac
      541 gaaggtccaa gtccaagtcc tcgtcggtct ccagatctcg ttcgcggtcc aggtcccggt
      601 ctcggtccag gagtcctccc ccagtgtcca agagggaatc caaatccagg tcgcgatcga
      661 agagtccccc caagtctcct gaagaggaag gagcggtgtc ctctggcggc gactacaaag
      721 acgatgacga caaggagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
      781 ccggccctat ggtgagcaag ggcgaggagg ataacatggc catcatca
//
