LOCUS       sequence-162789-        3765 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing NPDC1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_NPDC1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(515..614)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(631..650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(632..650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(655..671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1102..1893
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1986..2574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2475..2494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2728..2745
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2904..2931)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3023..3109)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3158..3180
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3172..3189
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3173..3189
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3205..3304
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccccacacct ccctgggctc ccctgtgtca tccgacccgg tgcacatgtc gcccctggag
       61 ccccggggag ggcaaggcga cggcctcgcc cttgtgctga tcctggcgtt ctgtgtggcc
      121 ggtgcagccg ccctctccgt agcctccctc tgctggtgca ggctgcagcg tgagatccgc
      181 ctgactcaga aggccgacta cgccactgcg aaggcccctg gctcacctgc agctccccgg
      241 atctcgcctg gggaccagcg gctggcacag agcgcggaga tgtaccacta ccagcaccaa
      301 cggcaacaga tgctgtgcct ggagcggcat aaagagccac ccaaggagct ggacacggcc
      361 tcctcggatg aggagaatga ggacggagac ttcacggtgt acgagtgccc gggcctggcc
      421 ccgaccgggg aaatggaggt gcgcaaccct ctgttcgacc acgccgcact gtccgcgccc
      481 ctgccggccc ccagctcacc gcctgcactg ccatgcccaa ctttcttgta caaagttggc
      541 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      601 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
      661 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
      721 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
      781 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
      841 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
      901 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      961 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1021 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1081 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1141 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1201 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     1261 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1321 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     1381 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     1441 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     1501 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     1561 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     1621 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1681 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1741 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1801 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     1861 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     1921 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2641 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2701 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2761 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2821 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     2881 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     2941 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3001 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3061 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3121 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3181 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     3241 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagtt
     3301 ggcatggcga cgccgctgcc tccgccctcc ccgcggcacc tgcggctgct gcggctgctg
     3361 ctctccggcc tcgtcctcgg cgccgccctg cgtggagccg ccgccggcca cccggatgta
     3421 gccgcctgtc ccgggagcct ggactgtgcc ctgaagaggc gggcaaggtg tcctcctggt
     3481 gcacatgcct gtgggccctg ccttcagccc ttccaggagg accagcaagg gctctgtgtg
     3541 cccaggatgc gccggcctcc aggcgggggc cggccccagc ccagactgga agatgagatt
     3601 gacttcctgg cccaggagct tgcccggaag gagtctggac actcaactcc gcccctaccc
     3661 aaggaccgac agcggctccc ggagcctgcc accctgggct tctcggcacg ggggcagggg
     3721 ctggagctgg gcctcccctc cactccagga acccccacgc ccacg
//
