LOCUS       sequence-162933-        2505 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression of LFn-Fla fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, 
            Kazmierczak BI, Vance RE
  TITLE     NAIP proteins are required for cytosolic detection of specific 
            bacterial ligands in vivo.
  JOURNAL   J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub
            2016 Apr 4.
  PUBMED    27045008
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20151809"; journalName: "J Exp Med"; date: "2016-05-2- 
            2"; volume: "213"; issue: "5"; pages: "657-65"
COMMENT     Sequence Label: pET15b LFn-Fla
FEATURES             Location/Qualifiers
     source          1..2505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     130..149
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        130..148
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    149..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             188..210
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             229..246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             256..273
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
       61 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
      121 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
      181 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
      241 catcacagca gcggcctggt gccgcgcggc agccatatgg cgggcggtca tggtgatgta
      301 ggtatgcacg taaaagagaa agagaaaaat aaagatgaga ataagagaaa agatgaagaa
      361 cgaaataaaa cacaggaaga gcatttaaag gaaatcatga aacacattgt aaaaatagaa
      421 gtaaaagggg aggaagctgt taaaaaagag gcagcagaaa agctacttga gaaagtacca
      481 tctgatgttt tagagatgta taaagcaatt ggaggaaaga tatatattgt ggatggtgat
      541 attacaaaac atatatcttt agaagcatta tctgaagata agaaaaaaat aaaagacatt
      601 tatgggaaag atgctttatt acatgaacat tatgtatatg caaaagaagg atatgaaccc
      661 gtacttgtaa tccaatcttc ggaagattat gtagaaaata ctgaaaaggc actgaacgtt
      721 tattatgaaa taggtaagat attatcaagg gatattttaa gtaaaattaa tcaaccatat
      781 cagaaatttt tagatgtatt aaataccatt aaaaatgcat ctgattcaga tggacaagat
      841 cttttattta ctaatcagct taaggaacat cccacagact tttctgtaga attcttggaa
      901 caaaatagca atgaggtaca agaagtattt gcgaaagctt ttgcatatta tatcgagcca
      961 cagcatcgtg atgttttaca gctttatgca ccggaagctt ttaattacat ggataaattt
     1021 aacgaacaag aaataaatct atccttggaa gaacttaaag atcaacggag cggccgcatg
     1081 gctcaagtaa tcaacactaa tgtggcgtcg ctcacagccc aacgtaattt gggtgtttcg
     1141 ggcaacatga tgcaaacatc gatccagcgt ttatcatcgg gattaaggat taacagtgcc
     1201 aaagatgatg cagcaggact cgccatttct caacgaatga ctgcacaaat tcgcgggatg
     1261 aaccaagccg ttagaaatgc caacgatggt atttccctgg ctcaggttgc tgaaggagca
     1321 atgcaggaaa caaccaatat tttacaacgc atgcgtgagc tttccgttca agctgctaac
     1381 tcaaccaata actcatctga ccggtcttca attcaaagtg aaatttcaca attaaaaagt
     1441 gaattggagc gtattgctca aaatactgaa ttcaatggtc aaagaattct cgatggttct
     1501 ttctctggcg caagcttcca ggttggggcg aattcaaacc aaaccatcaa tttcagtatc
     1561 ggcagcacaa aagcttcttc tcttggtggt attgccacgg caacaggaac agaagtagca
     1621 ggtgcagcag cggcagatat tactatcgca attggaggag gagcagcaac cagtattaac
     1681 tcttctgcca attttaccgg ggcactaaac ggacaagatg ctacgtcagc ctatgccaaa
     1741 gcagcggcta tcaacgatgc cgggatagga ggactatcgg ttacagcgtc taccagcggc
     1801 acacaagcag ttggcgcaat aggtggaact gcaggtgata cctacaactt aacgattaat
     1861 ggtgttgcaa tatacactaa ccttaacgta gcaacggcat taaccaactc tgatcttaga
     1921 gatgccatca atggcgtcag taaccaaact ggagttgttg catcacttaa cggtggtaat
     1981 atgacactca ctgctgctga tggcagaaac attacggtaa cagaaagcgg tacaggtttt
     2041 actgcaggta ctgacggttt aactgtaact ggtggcgcat ttgatggcgc gctacgcggt
     2101 actttatcaa tcagcgccgt tgatactatc gcgattggtg gtactgttgc taacattggt
     2161 ttaagtgcca atatctctaa agacacagta gggatagatt cactggatgt gagcacagcg
     2221 tctggcgctc aaacagcgat taagagaatt gatgcagcac tcaatagcgt aaactctaat
     2281 cgggctaata tgggtgctct gcaaaatcgc tttgaatcaa cgatagccaa cctgcaaaac
     2341 gtatcagaca atttatcagc tgctagaagt cgtattcaag atgctgatta cgctgctgaa
     2401 atggctagtt tgaccaaaaa ccaaattctg caacaagcag gtacagcgat gttggcacaa
     2461 gctaatagcc taccgcaatc tgtattatct ttgttaggtc gatag
//
