LOCUS       sequence-162945-        3117 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing GTF2A2, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GTF2A2_WT_V5
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(13..112)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(129..148)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(130..148)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(153..169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             600..1391
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1484..2072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1973..1992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2226..2243
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2402..2429)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2521..2607)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2656..2678
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2670..2687
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2671..2687
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2703..2802
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atactacaga atgcccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
       61 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
      121 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      181 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
      241 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
      301 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
      361 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      421 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      481 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      541 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
      601 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      661 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      721 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      781 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      841 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      901 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      961 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1021 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1081 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1141 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1201 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1261 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1321 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     1381 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     1441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2161 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2221 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2281 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2341 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     2401 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     2461 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     2521 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     2581 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     2641 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     2701 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
     2761 aatgcttttt tataatgcca actttgtaca aaaaagttgg catggcatat cagttataca
     2821 gaaatactac tttgggaaac agtcttcagg agagcctaga tgagctcata cagtctcaac
     2881 agatcacccc ccaacttgcc cttcaagttc tacttcagtt tgataaggct ataaatgcag
     2941 cactggctca gagggtcagg aacagagtca atttcagggg ctctctaaat acgtacagat
     3001 tctgcgataa tgtgtggact tttgtactga atgatgttga attcagagag gtgacagaac
     3061 ttattaaagt ggataaagtg aaaattgtag cctgtgatgg taaaaatact ggctcta
//
