LOCUS       sequence-162958-         810 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Empty entry vector for cloning inducible mammalian expression and an
            in-frame N-terminal 3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pEN_TT miRc2 3xFLAG
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..182
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(221..347)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(368..673)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(470..489)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
       61 ggccagagct gcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc
      121 gttgcaacaa attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg
      181 gtctagatat ctcgaaaacg cattagtctt ccaattgaaa aaagtgattt aatttatacc
      241 attttaattc agctttgtaa aaatgtatca aagagatagc aaggtattca gttttagtaa
      301 acaagataat tgctcctaaa gtagcccctt gaattccgag gcagtaggca agcagcaggt
      361 tggaattcta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
      421 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
      481 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggagactt
      541 tatctgacag cagacgtgca ctggccaggg ggatcactat ccgtcgcccg ggcgtgtcaa
      601 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa
      661 ccttaaactg cattctggaa gcacacctca agcttacctg cagcacgctc actgtcaaca
      721 gcaatatacc ttctcgagcc ttctgttggg ttaacctgaa gaagtaatcc cagcaagtgt
      781 ttccaagatg tgcaggcaac gattctgtaa
//
