LOCUS       sequence-163181-         836 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Co-expresses a hisx6 tagged copy of the Ivy protein and the wild 
            type human lysozyme protein, both in the cytoplasmic compartment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRSF-Ivy-his-hLYZ
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    477..501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(501..520)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(502..520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        566..643
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(663..682)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gcattatgcg gccgcaagct tagtggtgat gatggtgatg tttaaaatta aagccatccg
       61 gatggttttc caggctgccg gtcaacgccg cgaacaacac cgttttacca tcaatcgaaa
      121 gcgcatcgtt cacattcagc caggtgagtt tctcttgcga cgttttctca tcaatagtcg
      181 agaacagccc cgtcatctga ttagatttct cggaccacat cacagcgata cgttgcgagc
      241 cacagtcatg cggtttgcac gcgctcatca cctgatacgt ctcatctccc aacgttacgg
      301 tttgtgcggg agtataagta ccgcctttca tcacccaggc aggcagctta tgcccttgta
      361 ccatctgatt aaatgcagct ttggtggttt cgccctttgc aaggctgcta atggttaaat
      421 catcctgccc catggtatat ctccttatta aagttaaaca aaattatttc tacaggggaa
      481 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcctaat gcaggagtcg
      541 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
      601 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
      661 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
      721 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
      781 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattg
//
