LOCUS       sequence-163395-         975 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP-Epn1 (18-157) Dr in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub22-Epn1 (18-157) Dr
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(439..462)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             871..882
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(892..917,918..923,924..940,941..946,
                     947..965))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    922..940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    947..965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 actagttcag gtctgtgcca tcttctcttt ggtcttgagt gcatggatcc tctcctctcg
       61 tagcctctcc tcgtctttta gcagagtcac cagttgcttg gccttttccc gtacattcac
      121 accctggtct ttgccgtctc tgtctatgta ctggaagtct ttaagcgttt gaacggcgta
      181 gatgttttcc ctgcattgct gtgctacccg ctctgaaccc gtctttatca gatactccat
      241 cagtgtcata gctttatata catgtctcca gtttttccca tggtcgttga gtctcttcca
      301 gaccatgctc ataatctctg agaaggccac tacattgtat gtcaggtcag cgatctctga
      361 catcagagaa cttgagggac cccatggatc attagaagtt gcttccctta cctttatctc
      421 cgcctctgac atccgcgggg gcccctggaa cagaacttcc agggtcgtat cgatccccat
      481 ggggtgatga tggtgatggc tgctgcccat ctgcagggcg cgcctgccgc tcgaggcacc
      541 gcttgacccg ctgctgcctg cgctaccgcc gctgccagat cccgctccgc ttcccgagcc
      601 cgctgagccg ccggcgcgcc tgtctttctt ctcgtagact tcaaacttat acttgatgcc
      661 tttttcctcc tggacctcag agaggacgcc tgggtattct gggagaagtt tatatttccc
      721 caaatcaatt tctgggaaaa acgtgtcact ttcaaattcc tgcatgatcc ttgtcacaaa
      781 gagtctgagg tggcctggtt gattcatggc ttcctggtaa acagaactgc ctccgactat
      841 ccaaaccatg tctactttac tcatacgcgt tttctcctct ttaatgaatt cggtcagtgc
      901 gtcctgctga tgtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat
      961 agggactcga ggtga
//
