LOCUS       sequence-163760-         638 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the WTSI 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-WTSI
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(277..330)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 caggaggtcg ttgaactcct cggtctccct ccgggctagg agggccgggt tgttactgct
       61 gcaagctgca ccagctccgc cactctccag gtctgtggcc accgtcgccg ccaggtcgta
      121 ggggcggccg ggaagtgggg gaagtcgctt catgtgagag agttcctcac gccaacggtt
      181 agtcggggca cctgctggac gcagtgtctt ctcccttccc gccgggccgg acgcgaacgt
      241 ggagaaggac gggagcagag cgtcgctgac agccattggg ccaggattct cctccacgtc
      301 accgcatgtt agaagacttc ctctgccctc tcctccggac ccgccgccca tgtgcgacag
      361 gggcagtgtg ccgttaatgg ccgtgccggg caccgggccg ctctggtagt gctgggccat
      421 gtgcagtcta ctgggcgcag cgggctccgg cacctcggcg ccggggaggt acatgctgat
      481 catgtcccgg aggtccccgg cctggcaggg cgccctggag tgggaggaag aggtaaccac
      541 gggggggctg gagctggcct cggacttgac cacagagccc atggagccca gcgccatacc
      601 gggggtgccc tgctgcgagt aggacatgct gtaggtgg
//
