LOCUS       sequence-163808-        4467 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing TRAF5 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TRAF5_WT
FEATURES             Location/Qualifiers
     source          1..4467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1867..1966)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1983..2002)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1984..2002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2007..2023)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2007..2023)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2454..3245
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3338..3926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3827..3846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4080..4097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4256..4283)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4375..4461)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcttat tcagaagagc ataaaggtat gccctgtggt ttcatccgcc
      241 agaattccgg caactccatt tccttggact ttgagcccag tatagagtac cagtttgtgg
      301 agcggttgga agagcgctac aaatgtgcct tctgccactc ggtgcttcac aacccccacc
      361 agacaggatg tgggcaccgc ttctgccagc actgcatcct gtccctgaga gaattaaaca
      421 cagtgccaat ctgccctgta gataaagagg tcatcaaatc tcaggaggtt tttaaagaca
      481 attgttgcaa aagagaagtc ctcaacttat atgtatattg cagcaatgct cctggatgta
      541 atgccaaggt tattctgggc cggtaccagg atcaccttca gcagtgctta tttcaacctg
      601 tgcagtgttc taatgagaag tgccgggagc cagtcctacg gaaagacctg aaagagcatt
      661 tgagtgcatc ctgtcagttt cgaaaggaaa aatgccttta ttgcaaaaag gatgtggtag
      721 tcatcaatct acagaatcat gaggaaaact tgtgtcctga atacccagta ttttgtccca
      781 acaattgtgc gaagattatt ctaaaaactg aggtagatga acacctggct gtatgtcctg
      841 aagctgagca agactgtcct tttaagcact atggctgtgc tgtaacggat aaacggagga
      901 acctgcagca acatgagcat tcagccttac gggagcacat gcgtttggtt ttagaaaaga
      961 atgtccaatt agaagaacag atttctgact tacacaagag cctagaacag aaagaaagta
     1021 aaatccagca gctagcagaa actataaaga aacttgaaaa ggagttcaag cagtttgcac
     1081 agttgtttgg caaaaatgga agcttcctcc caaacatcca ggtttttgcc agtcacattg
     1141 acaagtcagc ttggctagaa gctcaagtgc atcaattatt acaaatggtt aaccagcaac
     1201 aaaataaatt tgacctgaga cctttgatgg aagcagttga tacagtgaaa cagaaaatta
     1261 ccctgctaga aaacaatgat caaagattag ccgttttaga agaggaaact aacaaacatg
     1321 atacccacat taatattcat aaagcacagc tgagtaaaaa tgaagagcga tttaaactgc
     1381 tggagggtac ttgctataat ggaaagctca tttggaaggt gacagattac aagatgaaga
     1441 agagagaggc ggtggatggg cacacagtgt ccatcttcag ccagtccttc tacaccagcc
     1501 gctgtggcta ccggctctgt gctagagcat acctgaatgg ggatgggtca gggagggggt
     1561 cacacctgtc cctatacttt gtggtcatgc gaggagagtt tgactcactg ttgcagtggc
     1621 cattcaggca gagggtgacc ctgatgcttc tggaccagag tggcaaaaag aacattatgg
     1681 agaccttcaa acctgacccc aatagcagca gctttaaaag acctgatggg gagatgaaca
     1741 ttgcatctgg ctgtccccgc tttgtggctc attctgtttt ggagaatgcc aagaacgcct
     1801 acattaaaga tgacactctg ttcttgaaag tggccgtgga cttaactgac ctggaggatc
     1861 tctagttgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1921 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     1981 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2041 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2101 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2161 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2221 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2281 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2341 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2401 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2461 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2521 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2581 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2641 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2701 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2761 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2821 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2881 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2941 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3001 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3061 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3121 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3181 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3241 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4141 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4201 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4261 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4321 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4381 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4441 ctgagccttt cgttttattt gatgcct
//
