LOCUS       sequence-164251-         933 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral expression of doxycycline-inducible dominant negative 
            Rac1 and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pSLIK DN Rac1
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    162..186
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(201..728,729..731,732..779))
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(812..836)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(833..871)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    883..901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agcgtccggg cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc
       61 gccgtgagga ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatcttg
      121 ggtgggttaa ttaaccatct ttatggctcg agatgttatc aaccactttg tacaagaaag
      181 ctgggtctag atatctcgag ttacaacagc aggcattttc tcttcctctt cttcacggga
      241 ggcgggcaga ggactgctcg gatcgcttcg tcaaacactg tcttgaggcc tcgctgtgtg
      301 agcgccgagc actccaggta ttttacagca ccaatctcct tagccatggc tagaccctgc
      361 ggataggtga tgggagtcag cttcttctcc ttcagtttct cgatcgtgtc tttatcatcc
      421 ctaagatcaa gtttagttcc cactaggatg atgggagtgt tgggacagtg gtgccgcacc
      481 tcaggatacc actttgcacg gacattttca aatgatgcag gactcacaag ggaaaagcaa
      541 attaagaaca catctgtttg cggataggat agggggcgta atctgtcata atcttcttgt
      601 ccagctgtat cccataagcc cagattcacc ggttttccat ctaccataac attggcagaa
      661 taattgtcaa agacagtagg gatatattct ccaggaaatg cattggttgt gtaactgatc
      721 agtaggcagt ttttacctac agctccgtct cccaccacca cacacttgat ggcctgcatg
      781 aattcggtac cggatccagt cgactagtgg cgatctgacg gttcactaaa cgagctctgc
      841 ttatataggc ctcccaccgt acacgcctac ctcgacatac gttctctatc actgataggg
      901 agtaaactcg acatacgttc tctatcactg ata
//
