LOCUS       sequence-164410-        1003 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Nedd4L-deltaC2 with His Xpress tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Itani OA, Stokes JB, Thomas CP
  TITLE     Nedd4-2 isoforms differentially associate with ENaC and regulate its
            activity.
  JOURNAL   Am J Physiol Renal Physiol. 2005 Aug;289(2):F334-46. Epub 2005 Apr 
            5.
  PUBMED    15814530
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Renal Physiol."; date: "2005-08"; volume: "289(2)"; pages: 
            "F334-46. Epub 2005 Apr 5"
COMMENT     Sequence Label: hNedd4-2 delta C2
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    108..270
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             283..300
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             304..336
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(340..348,349..363)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttagc gcagaggctt
      121 ggggcagccg agcggcagcc aggccccggc ccgggcctcg gttccagaag ggagaggagc
      181 ccgccaaggc gcgcaagaga gcgggctgcc tcgcagtccg agccggagag ggagcgcgag
      241 ccgcgccggc cccggacggc ctccgaaacc atggggggtt ctcatcatca tcatcatcat
      301 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      361 aaggtaccag gatccatgga gcgaccctat acatttaagg actttctcct cagaccaaga
      421 agtcataagt ctcgagttaa gggatttttg cgattgaaaa tggcctatat gccaaaaaat
      481 ggaggtcaag atgaagaaaa cagtgaccag agggatgaca tggagcatgg atgggaagtt
      541 gttgactcaa atgactcggc ttctcagcac caagaggaac ttcctcctcc tcctctgcct
      601 cccgggtggg aagaaaaagt ggacaattta ggccgaactt actatgtcaa ccacaacaac
      661 cggaccactc agtggcacag accaagcctg atggacgtgt cctcggagtc ggacaataac
      721 atcagacaga tcaaccagga ggcagcacac cggcgcttcc gctcccgcag gcacatcagc
      781 gaagacttgg agcccgagcc ctcggagggc ggggatgtcc ccgagccttg ggagaccatt
      841 tcagaggaag tgaatatcgc tggagactct ctcggtctgg ctctgccccc accaccggcc
      901 tccccaggat ctcggaccag ccctcaggag ctgtcagagg aactaagcag aaggcttcag
      961 atcactccag actccaatgg ggaacagttc agctctttga ttc
//
