LOCUS       sequence-164484-        9269 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant 
            (A74G/C773S/C810S) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p3R112C/T180CBM3
FEATURES             Location/Qualifiers
     source          1..9269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4984..5003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5110..5127
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             9113..9130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9183..9201)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9197..9244
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     5101 agcagcggcc tggtgccgcg cggcagccat atgatctacc tgaaatcttt cgaacgtaac
     5161 atccgcctga tcaatatgaa agtggtttac gatgatgttc gtgtgctgaa agatattatc
     5221 caggcgctgg cccgcctggt tgatgaagcc gtgctgaaat tcaaacagga tagcgttgaa
     5281 ctggtggcac tggatcgtgc gcatattagc ctgatctctg ttaacctgcc gcgcgaaatg
     5341 ttcaaagaat acgatgtgaa cgatgaattt aaattcggtt tcaacaccca gtacctgatg
     5401 aaaatcctga aagttgcaaa acgtaaagaa gccattgaaa tcgcaagtga aagcccggat
     5461 agtgtgatta tcaacattat cggcagcacc aatcgtgaat ttaacgtttg caatctggaa
     5521 gtgtctgaac aggaaattcc ggaaatcaac ctgcagttcg atattagtgc gacgatcagc
     5581 tctgatggtt ttaaaagtgc catcagcgaa gtttctaccg tgacggataa tgtggttgtg
     5641 gaaggtcacg aagatcgcat tctgatcaaa gcagaaggcg aaagcgaagt tgaagtggaa
     5701 ttttctaaag attgcggcgg tctgcaggat ctggaattta gtaaagaaag caaaaattct
     5761 tacagtgcgg aatacctgga tgatgttctg tctctgacga aactgagtga ttacgtgaaa
     5821 atcagcttcg gcaaccagaa accgctgcag ctgtttttca atatggaagg cggtggcaaa
     5881 gttacgtacc tgctggcgcc gaaagtggga ggtagcatga caattaaaga aatgcctcag
     5941 ccaaaaacgt ttggagagct taaaaattta ccgttattaa acacagataa accggttcaa
     6001 gctttgatga aaattgcgga tgaattagga gaaatcttta aattcgaggc gcctggtcgt
     6061 gtaacgcgct acttatcaag tcagcgtcta attaaagaag catgcgatga atcacgcttt
     6121 gataaaaact taagtcaagg ccttaaattt gtacgtgatt ttgcaggaga cgggttattt
     6181 acaagctgga cgcatgaaaa aaattggaaa aaagcgcata atatcttact tccaagcttc
     6241 agtcagcagg caatgaaagg ctatcatgcg atgatggtcg atatcgccgt gcagcttgtt
     6301 caaaagtggg agcgtctaaa tgcagatgag catattgaag taccggaaga catgacacgt
     6361 ttaacgcttg atacaattgg tctttgcggc tttaactatc gctttaacag cttttaccga
     6421 gatcagcctc atccatttat tacaagtatg gtccgtgcac tggatgaagc aatgaacaag
     6481 ctgcagcgag caaatccaga cgacccagct tatgatgaaa acaagcgcca gtttcaagaa
     6541 gatatcaagg tgatgaacga cctagtagat aaaattattg cagatcgcaa agcaagcggt
     6601 gaacaaagcg atgatttatt aacgcatatg ctaaacggaa aagatccaga aacgggtgag
     6661 ccgcttgatg acgagaacat tcgctatcaa attattacat tcttaattgc gggacacgaa
     6721 acaacaagtg gtcttttatc atttgcgctg tatttcttag tgaaaaatcc acatgtatta
     6781 caaaaagcag cagaagaagc agcacgagtt ctagtagatc ctgttccaag ctacaaacaa
     6841 gtcaaacagc ttaaatatgt cggcatggtc ttaaacgaag cgctgcgctt atggccaact
     6901 gctcctgcgt tttccctata tgcaaaagaa gatacggtgc ttggaggaga atatccttta
     6961 gaaaaaggcg acgaactaat ggttctgatt cctcagcttc accgtgataa aacaatttgg
     7021 ggagacgatg tggaagagtt ccgtccagag cgttttgaaa atccaagtgc gattccgcag
     7081 catgcgttta aaccgtttgg aaacggtcag cgtgcgtgta tcggtcagca gttcgctctt
     7141 catgaagcaa cgctggtact tggtatgatg ctaaaacact ttgactttga agatcataca
     7201 aactacgagc tggatattaa agaaacttta acgttaaaac ctgaaggctt tgtggtaaaa
     7261 gcaaaatcga aaaaaattcc gcttggcggt attccttcac ctagcactga acagtctgct
     7321 aaaaaagtac gcaaaaaggc agaaaacgct cataatacgc cgctgcttgt gctatacggt
     7381 tcaaatatgg gaacagctga aggaacggcg cgtgatttag cagatattgc aatgagcaaa
     7441 ggatttgcac cgcaggtcgc aacgcttgat tcacacgccg gaaatcttcc gcgcgaagga
     7501 gctgtattaa ttgtaacggc gtcttataac ggtcatccgc ctgataacgc aaagcaattt
     7561 gtcgactggt tagaccaagc gtctgctgat gaagtaaaag gcgttcgcta ctccgtattt
     7621 ggatgcggcg ataaaaactg ggctactacg tatcaaaaag tgcctgcttt tatcgatgaa
     7681 acgcttgccg ctaaaggggc agaaaacatc gctgaccgcg gtgaagcaga tgcaagcgac
     7741 gactttgaag gcacatatga agaatggcgt gaacatatgt ggagtgacgt agcagcctac
     7801 tttaacctcg acattgaaaa cagtgaagat aataaatcta ctctttcact tcaatttgtc
     7861 gacagcgccg cggatatgcc gcttgcgaaa atgcacggtg cgttttcaac gaacgtcgta
     7921 gcaagcaaag aacttcaaca gccaggcagt gcacgaagca cgcgacatct tgaaattgaa
     7981 cttccaaaag aagcttctta tcaagaagga gatcatttag gtgttattcc tcgcaactat
     8041 gaaggaatag taaaccgtgt aacagcaagg ttcggcctag atgcatcaca gcaaatccgt
     8101 ctggaagcag aagaagaaaa attagctcat ttgccactcg ctaaaacagt atccgtagaa
     8161 gagcttctgc aatacgtgga gcttcaagat cctgttacgc gcacgcagct tcgcgcaatg
     8221 gctgctaaaa cggtcagccc gccgcataaa gtagagcttg aagccttgct tgaaaagcaa
     8281 gcctacaaag aacaagtgct ggcaaaacgt ttaacaatgc ttgaactgct tgaaaaatac
     8341 ccggcgagcg aaatgaaatt cagcgaattt atcgcccttc tgccaagcat acgcccgcgc
     8401 tattactcga tttcttcatc acctcgtgtc gatgaaaaac aagcaagcat cacggtcagc
     8461 gttgtctcag gagaagcgtg gagcggatat ggagaatata aaggaattgc gtcgaactat
     8521 cttgccgagc tgcaagaagg agatacgatt acgtgcttta tttccacacc gcagtcagaa
     8581 tttacgctgc caaaagaccc tgaaacgccg cttatcatgg tcggaccggg aacaggcgtc
     8641 gcgccgttta gaggctttgt gcaggcgcgc aaacagctaa aagaacaagg acagtcactt
     8701 ggagaagcac atttatactt cggctgccgt tcacctcatg aagactatct gtatcaagaa
     8761 gagcttgaaa acgcccaaag cgaaggcatc attacgcttc ataccgcttt ttctcgcatg
     8821 ccaaatcagc cgaaaacata cgttcagcac gtaatggaac aagacggcaa gaaattgatt
     8881 gaacttcttg atcaaggagc gcacttctat atttgcggag acggaagcca aatggcacct
     8941 gccgttgaag caacgcttat gaaaagctat gctgacgttc accaagtgag tgaagcagac
     9001 gctcgcttat ggctgcagca gctagaagaa aaaggccgat acgcaaaaga cgtgtgggct
     9061 gggtaaggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca
     9121 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     9181 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     9241 tttgctgaaa ggaggaacta tatccggat
//
