LOCUS       sequence-164490-         891 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Single Integration Vector LEU - Cloning vector for single 
            integration into the LEU2 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVl
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        346..364
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    377..484
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     393..409
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     394..410
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(444..460)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(444..460)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(492..511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(493..511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(512..545)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttcga attaattaaa ttttctgtta cacctaactt tttgtgtggt
      121 gccctcctcc ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc
      181 cattagtatc aatttgctta cctgtattcc tttacatcct cctttttctc cttcttgata
      241 aatgtatgta gattgcgtat atagtttcgt ctaccctatg aacatattcc attttgtaat
      301 ttcgtgtcgt ttctattatg aatttcattt ataaagttta caggaaatta accctcacta
      361 aagggaacaa aagctgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
      421 tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga
      481 gctccaattc gccctatagt gagtcgtatt aataacttcg tatagcatac attatacgaa
      541 gttattatat gaagattgtt ctaccattca caactatatg aaaggccaca gaagaaaatt
      601 accttccatg tggatgacga tatgcgtagc atggaaatgt atcatgaata aaatattgtt
      661 cctcagtcaa gcacataccg gaaagacaac tgcgatttaa taagagcaag aaggtgataa
      721 aacctactca ctcaaatgcc tctattgtaa aggaaaggac aaccctcatt ccaaaattta
      781 aatttatcaa tattctcatg ctaatatctt ctttacctcc tctgctactg cgtctcctac
      841 ctctgttgtt gagtttgatc ctcctaagtc tcctgtcctt attcctgcgt c
//
