LOCUS       sequence-164495-         996 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a URA selection 
            marker, originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-c
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             52..768
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      812..977
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(867..891)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aaattaaccc tcactaaagg ggcatgcgcg gccgctctag aactaattaa catgtctaaa
       61 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
      121 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
      181 ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
      241 ttaacttggg gtgttcaatg tttttctaga tacccagatc atatgaaaca acatgacttt
      301 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
      361 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
      421 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
      481 atttataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg tatcaaagct
      541 aacttcaaaa ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa
      601 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttatccact
      661 caatctagat tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt
      721 gttactgctg ctggtattac ccatggtatt gatgaattgt acaaataagg atgctagcat
      781 tttaactcga gggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat
      841 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
      901 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
      961 atagtatgag gtcgctctta ttgaccacac ctctac
//
