LOCUS       sequence-164739-         889 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEEEH_04.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB285
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(458..487)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcctc attagcactg tttctgcatt tctttacaga
       61 aacgacgcag gtcttcttcg tcagaagagc agtaacggta ttcttcaccg ttgtcacctt
      121 caacacgaac gcaagaagag gtgatgtggc aacggcagcc gccaatctgt tcacggtggg
      181 cttcaatgat gtcgttgtct tccatgtcca ggtcttccgg cgcttgatct gcctggatgc
      241 gaataccgtc atacaggaaa cgcaggctat ccatttcctt gccctgacgt ttggcaaacg
      301 cttccatcag gcgacgcagc ggcgtggtct ttttgatctt aaagaaaatt tcagagctgc
      361 cgtcactaac tttcaggtta atatgggttt ccggtttcac ttccggcttg acttccggct
      421 ttgcttcttg gttgacttcc gaatcttgca ggctaccgtg gtggtggtgg tggtggtggt
      481 ggtggtgacc catcttagtt ttcagatcat ttttaacgct tttcacacca tctaccgctt
      541 tggcgatact ttcagcacgg tcactttgtg cctgagaatc gacggtaccg gagagctgaa
      601 ccacgccgtc ggtggtttca actttcacat gacgggaagg gacgatatcg tccgccagca
      661 gtttggcttt gatttcactg gtggtggcgg tgtcacccgc gtagcccttc accgagcctt
      721 ctttagcgtc gcgaacgtgc agtttgtcgc tgacagaggt caccccttca acgcctttcg
      781 ccactttcac tgcctcttcg gcctgggcct ggctttcaac gaaaccgctc agggtcacga
      841 ctttttgatc ggtttttaca gagatatcgg tgctcttgat gttgtcatg
//
