LOCUS       sequence-165184-        4362 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing ATG16L1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ATG16L1_WT
FEATURES             Location/Qualifiers
     source          1..4362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     487..506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     740..757
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(916..943)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1035..1121)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1170..1192
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1184..1201
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1185..1201
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1217..1316
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2889..2988)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3005..3024)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3006..3024)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3029..3045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3029..3045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3476..4267
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4360..4362,1..586)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
       61 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
      181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
      541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
      601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
      661 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      721 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
      781 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      841 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
      901 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
      961 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
     1021 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     1081 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
     1141 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
     1201 tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
     1261 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag ttggcaccat
     1321 ggcccaactg aggattaagc accaagagga actgactgaa ttacacaaga aacgtgggga
     1381 gttagctcaa ctggtgattg acctgaataa ccaaatgcag cggaaggaca gggagatgca
     1441 gatgaatgaa gcaaaaattg cagaatgttt gcagactatc tctgacctgg agacggagtg
     1501 cctagacctg cgcactaagc tttgtgacct tgaaagagcc aaccagaccc tgaaggatga
     1561 atatgatgcc ctgcagatca cttttactgc cttggaggga aaactgagga aaactacgga
     1621 agagaaccag gagctggtca ccagatggat ggctgagaaa gcccaggaag ccaatcggct
     1681 taatgcagag aatgaaaaag actccaggag gcggcaagcc cggctgcaga aagagcttgc
     1741 agaagcagca aaggaacctc taccagtcga acaggatgat gacattgagg tcattgtgga
     1801 tgaaacttct gatcacacag aagagacctc tcctgtgcga gccatcagca gagcagccac
     1861 taagcgactc tcgcagcctg ctggaggcct tctggattct atcactaata tctttgggag
     1921 acgctctgtc tcttccttcc cagtccccca ggacaatgtg gatactcatc ctggttctgg
     1981 taaagaagtg agggtaccag ctactgcctt gtgtgtcttc gatgcacatg atggggaagt
     2041 caacgctgtg cagttcagtc caggttcccg gttactggcc actggaggca tggaccgcag
     2101 ggttaagctt tgggaagtat ttggagaaaa atgtgagttc aagggttccc tatctggcag
     2161 taatgcagga attacaagca ttgaatttga tagtgctgga tcttacctct tagcagcttc
     2221 aaatgatttt gcaagccgaa tctggactgt ggatgattat cgattacggc acacactcac
     2281 gggacacagt gggaaagtgc tgtctgctaa gttcctgctg gacaatgcgc ggattgtctc
     2341 aggaagtcac gaccggactc tcaaactctg ggatctacgc agcaaagtct gcataaagac
     2401 agtgtttgca ggatccagtt gcaatgatat tgtctgcaca gagcaatgtg taatgagtgg
     2461 acattttgac aagaaaattc gtttctggga cattcgatca gagagcatag ttcgagagat
     2521 ggagctgttg ggaaagatta ctgccctgga cttaaaccca gaaaggactg agctcctgag
     2581 ctgctcccgt gatgacttgc taaaagttat tgatctccga acaaatgcta tcaagcagac
     2641 attcagtgca cctgggttca agtgcggctc tgactggacc agagttgtct tcagccctga
     2701 tggcagttac gtggcggcag gctctgctga gggctctctg tatatctgga gtgtgctcac
     2761 agggaaagtg gaaaaggttc tttcaaagca gcacagctca tccatcaatg cggtggcgtg
     2821 gtcgccctct ggctcgcacg ttgtcagtgt ggacaaagga tgcaaagctg tgctgtgggc
     2881 acagtacttg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2941 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3001 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3061 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     3121 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     3181 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     3241 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     3301 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     3361 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3421 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     3481 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3541 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3601 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3661 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3721 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3781 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3841 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3901 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3961 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     4021 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     4081 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4141 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4201 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     4261 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tg
//
