LOCUS       sequence-165477-         676 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  shRNA targeting BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pAd388-BPTF-sh28
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(123..371)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(181..200)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(351..371)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_signal     399..549
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 gcccacacat ttcagtacct caatcgatgg ccactcgtgg gtaccactag tgatatcgaa
       61 ttcacgcgta aaaaaagcac agagaagacc atgatctctt gaatcatggt cttctctgtg
      121 ctggatcctc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
      181 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
      241 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
      301 attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
      361 aataggccct cttcctgccc agatctccct agacaaatat tacgcgctat gagtaacaca
      421 aaattattca gatttcactt cctcttattc agttttcccg cgaaaatggc caaatcttac
      481 tcggttacgc ccaaatttac tacaacatcc gcctaaaacc gcgcgaaaat tgtcacttcc
      541 tgtgtacacc ggcgcacacc aaaaacgtca cttttgccac atccgtcgct tacatgtgtt
      601 ccgccacact tgcaacatca cacttccgcc acactactac gtcacccgcc ccgttcccac
      661 gccccgcgcc acgtca
//
