LOCUS       sequence-165519-         910 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Chain B of the habu snake venom FIX/FX binding protein with 
            C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' 
            NheI and 3' BamHI; IRES vector conferring puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Robert Peters
  TITLE     rFIX/X-binding protein
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: rFIXX-bp chain B (IRESpuro)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatcgatatc tgcggcctag ctagccgcca ccatggggcg
      121 attcatcttc atgagctttg gcttcctggt cgtgttcctc tccctgagtg gaactgcagc
      181 tgattgtccc tctgattggt cctcttatga agggcattgc tacaagccct tcagtgaacc
      241 caaaaactgg gccgatgcag agaatttctg cacacaacag cacgcaggcg gacatctggt
      301 ctccttccaa agcagtgaag aagcagattt tgtggtcaag ctggccttcc aaacttttgg
      361 ccacagtatt ttctggatgg gactgagcaa tgtctggaat caatgcaact ggcagtggag
      421 caatgctgcc atgctcagat acaaagcctg ggctgaagaa tcttattgtg tctatttcaa
      481 gtcaactaat aacaaatgga ggagtagagc ctgcagaatg atggcacagt tcgtctgcga
      541 gttccaggca ggaggaggca gcggaggagg gtctttgaac gacatttttg aagcgcaaaa
      601 aattgaatgg cattgaggat ccgcggccgc atagataact gatccagtgt gctggaatta
      661 attcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga
      721 cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg
      781 cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt
      841 caagcttgag gtgtggcagg cttgagatct ggccatacac ttgagtgaca atgacatcca
      901 ctttgccttt
//
