LOCUS       sequence-165725-        4977 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing SV2C , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SV2C_p.M727I
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2377..2476)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2493..2512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2494..2512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2517..2533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2517..2533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2964..3755
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3848..4436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4337..4356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4590..4607
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4766..4793)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4885..4971)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggaagac tcttacaagg ataggacttc actgatgaag ggtgccaagg
      241 acattgccag agaggtgaag aaacaaacag taaagaaggt gaatcaagct gtggaccgag
      301 cccaggatga atacacccag aggtcctaca gtcggttcca agatgaagaa gatgatgatg
      361 actactaccc ggctggagaa acctataatg gtgaggccaa cgatgacgaa ggctcaagtg
      421 aagccactga ggggcatgat gaagatgatg agatctatga gggggagtat cagggcatcc
      481 ccagtatgaa ccaagcgaag gacagcatcg tgtcagtggg gcagcccaag ggcgatgagt
      541 acaaggaccg acgggagctg gaatcagaaa ggagagctga cgaggaagag ttagcccagc
      601 agtatgagct gataatccaa gaatgcggtc atggtcgttt tcagtgggcc cttttcttcg
      661 tcctgggcat ggctcttatg gcagacggtg tagaggtgtt tgtcgttggc ttcgtgttac
      721 ccagtgctga gacagacctc tgcatcccaa attcaggatc tggatggcta ggcagcatag
      781 tgtacctcgg gatgatggtg ggggcgttct tctggggagg actggcagac aaagtgggaa
      841 ggaaacagtc tcttctgatt tgcatgtctg tcaacggatt ctttgccttc ctttcttcat
      901 ttgtccaagg ttatggcttc tttctcttct gtcgcttact ttctggattc gggattggag
      961 gagccatacc cactgtgttc tcgtactttg ctgaagtcct ggcccgggaa aagcggggcg
     1021 aacacttgag ctggctctgc atgttctgga tgatcggtgg catctacgcc tctgccatgg
     1081 cctgggccat catcccgcac tacgggtgga gcttcagcat gggatcggcc taccagtttc
     1141 acagttggcg tgtgtttgtc atcgtctgtg cactcccctg tgtctcctcc gtggtggccc
     1201 tcacattcat gcctgaaagc ccacgatcct tgttggaggt tggaaaacat gatgaagctt
     1261 ggatgattct gaagttaatt catgacacca acatgagagc ccggggtcag cctgagaagg
     1321 tcttcacggt aaacaaaata aaaactccta aacaaataga tgagctgatt gaaattgaga
     1381 gtgacacagg aacatggtat aggaggtgtt ttgttcggat ccgcaccgag ctgtacggaa
     1441 tttggttgac ttttatgaga tgtttcaact acccagtcag ggataataca ataaagctta
     1501 caattgtttg gttcaccctg tcctttgggt actatggatt atccgtttgg ttccctgatg
     1561 tcattaaacc tctgcagtcc gatgaatatg cattgctaac cagaaatgtg gagagagata
     1621 aatatgcaaa tttcactatt aactttacaa tggaaaatca gattcatact ggaatggaat
     1681 acgacaatgg cagattcata ggggtcaagt tcaaatctgt aactttcaaa gactctgttt
     1741 ttaagtcctg cacctttgag gatgtaactt cagtgaacac ctacttcaag aactgcacat
     1801 ttattgacac tgtttttgac aacacagatt ttgagccata taaattcatt gacagtgaat
     1861 ttaaaaactg ctcgtttttt cacaacaaga cgggatgtca gattaccttt gatgatgact
     1921 atagtgccta ctggatttat tttgtcaact ttctggggac attggcagta ttgccaggga
     1981 acattgtgtc tgctctgctg atggacagaa ttgggcgctt aacaatgcta ggtggctcta
     2041 tggtgctttc ggggatcagc tgtttcttcc tttggttcgg caccagtgaa tccatgatga
     2101 taggcatgct gtgtctgtac aatggattga ccatctcagc ctggaactct cttgacgtgg
     2161 tcactgtgga actgtacccc acagaccgga gggcaacagg ctttggcttc ttaaatgcgc
     2221 tatgcaaggc agcagccgtc ctgggaaact taatatttgg ctctctggtc agcatcacca
     2281 aatcaatccc catcctgctg gcttctactg tgctcgtgtg tggaggactc gttgggctgt
     2341 gcctgcctga cacacgaacc caggttctga tttgattgcc aactttcttg tacaaagttg
     2401 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2461 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     2521 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2581 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2641 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2701 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2761 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2821 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2881 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2941 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     3001 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3061 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3121 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3181 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3241 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3301 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3361 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3421 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3481 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3541 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3601 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3661 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3721 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3781 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3841 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3901 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3961 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4021 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4081 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4141 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4201 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4261 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4321 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4381 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4441 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4501 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4561 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4621 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4681 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4741 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4801 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4861 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4921 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcct
//
