LOCUS       sequence-166262-       12746 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of MYB after doxycycline administration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, 
            Bernstein ID, Collins JJ, Zon LI, Daley GQ
  TITLE     Induction of multipotential hematopoietic progenitors from human 
            pluripotent stem cells via respecification of lineage-restricted 
            precursors.
  JOURNAL   Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 
            10.1016/j.stem.2013.09.002.
  PUBMED    24094326
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.09"; journalName: "Cell Stem Cell"; date: 
            "2013-10-3- 3"; volume: "13"; issue: "4"; pages: "459-70"
COMMENT     Sequence Label: pINDUCER21-MYB
FEATURES             Location/Qualifiers
     source          1..12746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(266..271,272..289,290..296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     328..344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     328..344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     394..413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             605..1238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1285..1410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1907..2140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2325..2369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2662..2779
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2834..2852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2876..2894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2960..2978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3011..3049
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3046..3070
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3179..3203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5523..5547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5600..5626
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5846..7024
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6077..7015
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6972..6992
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7094..7127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7134..7751,7752..7757,7758..7793,7794..7796,7797..7832,
                     7833..7835,7836..7871,7872..7880)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7937..8510
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8104..8121)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8331..8350
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8526..8528,8529..8531,8532..9245)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8526..8528,8529..8531,8532..9242)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8571..8592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8832..8851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9179..9200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9381..9400
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9688..10276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9741..9761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10159..10170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10351..10584
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10703..10721)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10789..10893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10894..10962,10963..11754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11112..11131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11925..12513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12414..12433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12667..12684
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
       61 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
      181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
      361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
      541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      601 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      661 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      721 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      781 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      841 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      901 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      961 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     1021 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     1081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1741 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2641 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2701 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2761 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2821 gatcctttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2881 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2941 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta
     3001 cccgggtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     3061 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     3121 ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt ggaattctgc agatatcaac
     3181 aagtttgtac aaaaaagcag gcttgaagga attcggtacc atggcccgaa gaccccggca
     3241 cagcatatat agcagtgacg aggatgatga ggactttgag atgtgtgacc atgactatga
     3301 tgggctgctt cccaagtctg gaaagcgtca cttggggaaa acaaggtgga cccgggaaga
     3361 ggatgaaaaa ctgaagaagc tggtggaaca gaatggaaca gatgactgga aagttattgc
     3421 caattatctc ccgaatcgaa cagatgtgca gtgccagcac cgatggcaga aagtactaaa
     3481 ccctgagctc atcaagggtc cttggaccaa agaagaagat cagagagtga tagagcttgt
     3541 acagaaatac ggtccgaaac gttggtctgt tattgccaag cacttaaagg ggagaattgg
     3601 aaaacaatgt agggagaggt ggcataacca cttgaatcca gaagttaaga aaacctcctg
     3661 gacagaagag gaagacagaa ttatttacca ggcacacaag agactgggga acagatgggc
     3721 agaaatcgca aagctactgc ctggacgaac tgataatgct atcaagaacc actggaattc
     3781 tacaatgcgt cggaaggtcg aacaggaagg ttatctgcag gagtcttcaa aagccagcca
     3841 gccagcagtg gccacaagct tccagaagaa cagtcatttg atgggttttg ctcaggctcc
     3901 gcctacagct caactccctg ccactggcca gcccactgtt aacaacgact attcctatta
     3961 ccacatttct gaagcacaaa atgtctccag tcatgttcca taccctgtag cgttacatgt
     4021 aaatatagtc aatgtccctc agccagctgc cgcagccatt cagagacact ataatgatga
     4081 agaccctgag aaggaaaagc gaataaagga attagaattg ctcctaatgt caaccgagaa
     4141 tgagctaaaa ggacagcagg tgctaccaac acagaaccac acatgcagct accccgggtg
     4201 gcacagcacc accattgccg accacaccag acctcatgga gacagtgcac ctgtttcctg
     4261 tttgggagaa caccactcca ctccatctct gccagcggat cctggctccc tacctgaaga
     4321 aagcgcctcg ccagcaaggt gcatgatcgt ccaccagggc accattctgg ataatgttaa
     4381 gaacctctta gaatttgcag aaacactcca atttatagat tctgattctt catcatggtg
     4441 tgatctcagc agttttgaat tctttgaaga agcagatttt tcacctagcc aacatcacac
     4501 aggcaaagcc ctacagcttc agcaaagaga gggcaatggg actaaacctg caggagaacc
     4561 tagcccaagg gtgaacaaac gtatgttgag tgagagttca cttgacccac ccaaggtctt
     4621 acctcctgca aggcacagca caattccact ggtcatcctt cgaaaaaaac ggggccaggc
     4681 cagcccctta gccactggag actgtagctc cttcatattt gctgacgtca gcagttcaac
     4741 tcccaagcgt tcccctgtca aaagcctacc cttctctccc tcgcagttct taaacacttc
     4801 cagtaaccat gaaaactcag acttggaaat gccttcttta acttccaccc ccctcattgg
     4861 tcacaaattg actgttacaa caccatttca tagagaccag actgtgaaaa ctcaaaagga
     4921 aaatactgtt tttagaaccc cagctatcaa aaggtcaatc ttagaaagct ctccaagaac
     4981 tcctacacca ttcaaacatg cacttgcagc tcaagaaatt aaatacggtc ccctgaagat
     5041 gctacctcag acaccctctc atctagtaga agatctgcag gatgtgatca aacaggaatc
     5101 tgatgaatct ggaattgttg ctgagtttca agaaaatgga ccacccttac tgaagaaaat
     5161 caaacaagag gtggaatctc caactgataa atcaggaaac ttcttctgct cacaccactg
     5221 ggaaggggac agtctgaata cccaactgtt cacgcagacc tcgcctgtgg cagatgcacc
     5281 gaatattctt acaagctccg ttttaatggc accagcatca gaagatgaag acaatgttct
     5341 caaagcattt acagtaccta aaaacaggtc cctggcgagc cccttgcagc cttgtagcag
     5401 tacctgggaa cctgcatcct gtggaaagat ggaggagcag atgacatctt ccagtcaagc
     5461 tcgtaaatac gtgaatgcat tctcagcccg gacgctggtc atgtagctcg agtgcggccg
     5521 caacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
     5581 agagggcccg cggttcgaat acccatacga cgtcccagac tacgcttagt aatgattaat
     5641 taaactagaa attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagtaaccc
     5701 acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     5761 ggcgagccgc gtaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
     5821 ctcctcacgg cgagcctagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
     5881 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     5941 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     6001 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     6061 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     6121 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     6181 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     6241 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     6301 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     6361 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     6421 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     6481 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     6541 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     6601 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     6661 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     6721 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     6781 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     6841 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     6901 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     6961 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     7021 gtgaagcggc cgccatgcta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     7081 gttagacgga tccataactt cgtataatgt atgctatacg aagttatgcc accatgtcta
     7141 gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg
     7201 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     7261 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     7321 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     7381 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     7441 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     7501 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     7561 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     7621 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
     7681 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     7741 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     7801 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     7861 ttgacatgct ccccgggtaa ctaagtaagg atccgcggcc gcactagagg aattccgccc
     7921 ctctcccccc cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     7981 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     8041 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     8101 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     8161 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     8221 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     8281 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     8341 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     8401 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     8461 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttaccggt
     8521 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     8581 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     8641 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     8701 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     8761 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     8821 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8881 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8941 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     9001 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     9061 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     9121 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     9181 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     9241 agtaagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     9301 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     9361 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     9421 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     9481 cttctatcgc cttcttgacg agttcttctg atgtacaagt aaagcggccg cgactctaga
     9541 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9601 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc
     9661 gatatcaagc ttatcgcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     9721 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     9781 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9841 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9901 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9961 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
    10021 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
    10081 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
    10141 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
    10201 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
    10261 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
    10321 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    10381 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10441 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10501 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10561 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
    10621 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
    10681 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
    10741 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
    10801 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10861 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    10921 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    10981 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    11041 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    11101 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    11161 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    11221 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    11281 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    11341 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    11401 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    11461 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    11521 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    11581 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    11641 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    11701 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    11761 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11821 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    11881 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    11941 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    12001 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    12061 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    12121 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    12181 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    12241 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    12301 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    12361 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    12421 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    12481 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    12541 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    12601 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    12661 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    12721 cccgcgcgtt ggccgattca ttaatg
//
