LOCUS       sequence-166350-        3858 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing HAPLN1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HAPLN1_p.K115N
FEATURES             Location/Qualifiers
     source          1..3858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     421..438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(597..624)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(716..802)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     851..873
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     865..882
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     866..882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    898..997
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2066..2165)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2182..2201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2183..2201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2206..2222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2206..2222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2653..3444
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3537..3858,1..267)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      541 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
      601 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
      661 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
      721 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
      781 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
      841 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
      901 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
      961 ttttttataa tgccaacttt gtacaaaaaa gttggcacca tgaagagtct acttcttctg
     1021 gtgctgattt caatctgctg ggctgatcat ctttcagaca actatactct ggatcatgac
     1081 agagctattc acatccaagc agaaaatggc ccccatctac ttgtggaagc agagcaagcc
     1141 aaggtgtttt cacacagagg tggcaatgtt acactgccat gtaaatttta tcgagaccct
     1201 acagcatttg gctcaggaat ccataaaatc cgaattaagt ggaccaagct aacttcggat
     1261 tacctcaagg aagtggatgt ttttgtttcc atgggatacc acaaaaaaac ctatggaggc
     1321 taccagggta gagtgtttct gaacggaggc agtgatagtg atgcttctct ggtcatcaca
     1381 gacctcactc tggaagatta tgggagatat aagtgtgagg tgattgaagg attagaagat
     1441 gatactgttg tggtagcact ggacttacaa ggtgtggtat tcccttactt tccacgactg
     1501 gggcgctaca atctcaattt tcacgaggcg cagcaggcgt gtctggacca ggatgctgtg
     1561 atcgcctcct tcgaccagct gtacgacgcc tggcggggcg ggctggactg gtgcaatgcc
     1621 ggctggctca gtgatggctc tgtgcaatat cccatcacaa agcccagaga gccctgtggg
     1681 ggccagaaca cagtgcccgg agtcaggaac tacggatttt gggataaaga taaaagcaga
     1741 tatgatgttt tctgttttac atccaatttc aatggccgtt tttactatct gatccacccc
     1801 accaaactga cctatgatga agcggtgcaa gcttgtctca atgatggtgc tcagattgca
     1861 aaagtgggcc agatatttgc tgcctggaaa attctcggat atgaccgctg tgatgcgggc
     1921 tggttggcgg atggcagcgt ccgctacccc atctctaggc caagaaggcg ctgcagtcct
     1981 actgaggctg cagtgcgctt cgtgggtttc ccagataaaa agcataagct gtatggtgtc
     2041 tactgcttca gagcatacaa ctgattgcca actttcttgt acaaagttgg cattataaga
     2101 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2161 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2221 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2281 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     2341 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2401 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2461 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2521 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2581 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2641 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2701 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2761 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     2821 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2881 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2941 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     3001 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     3061 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3121 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3181 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3241 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3301 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3361 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3421 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     3481 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3541 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3601 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3661 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3841 cagcggtcgg gctgaacg
//
