LOCUS       sequence-166409-         992 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  N-Flag/HA tagged HPV 197 E7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Grace M, Munger K
  TITLE     Proteomic analysis of the gamma human papillomavirus type 197 E6 and
            E7 associated cellular proteins.
  JOURNAL   Virology. 2016 Oct 20;500:71-81. doi: 10.1016/j.virol.2016.10.010.
  PUBMED    27771561
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2016-10-20"; volume: "500:71-81. doi"; pages: " 
            10.1016/j.virol.2016.10.010"
COMMENT     Sequence Label: CMV-N 197 E7
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(616..635)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    681..736
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(735..754)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
       61 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
      121 gtctcatcat tttggcaaag aattcctcga cggatctatg gactacaagg atgacgatga
      181 caagctcgat ggaggatacc catacgatgt tccagattac gctgatatcg gatccatgca
      241 cggggaaaga gcaactatta gagatgtggt cctggaactg acaaaggtcg cctgtaactc
      301 agaagtcctg tttgaagaga gcctgagctc cgactccgag gaagagcacg aaatcttcaa
      361 gattgagacc cgatgcgata actgtcgaag aggcatcagg gtgtgcgtcc gcgcatctaa
      421 tcaggggatt agtagactgc agtggctgct ggaacatgac attaggtttc tgtgccccgc
      481 ttgtagccgc aatctgtttc agcatggaag gtttcagtaa ggatccgtcg aggaattcac
      541 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      601 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
      661 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
      721 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
      781 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
      841 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
      901 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
      961 tttaacatcc ctaaaatttt ccttacatgt tt
//
