LOCUS       sequence-166431-         986 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADNT-kan
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(315..361)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(458..478)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(491..508)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(545..829)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(603..622)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 agatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
       61 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      121 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      181 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      241 tgcagtttca tttgatgctc gatgagtttt tctaatcagc gccccctcta gctgatcacc
      301 gcggccatga tggcataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      361 gatgcctggc agtcgatcgt acgctagtta actacgtcga catcgataag cttgctagcg
      421 atatcgaatt cggatccggt accttatcgt ccatggcgcc ctggaagtac aggttctcgc
      481 cggccgacgg gtggtggtgg tggtggtgca tggttaattc ctcctgttag cccaaaaaac
      541 gggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt
      601 atggataaaa atgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtggact
      661 tttctgccgt gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt
      721 tgttacagaa tgcttttaat aagcggggtt accggtttgg ttagcgagaa gagccagtaa
      781 aagacgcagt gacggcaatg tctgatgcaa tatggacaat tggtttcttc tctgaatggc
      841 gggagtatga aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt
      901 aatgcccatc tggtggcggg tttaacgccg attgaggcca acggttatct cgattttttt
      961 atcgaccgac cgctgggaat gaaagg
//
