LOCUS       sequence-166448-         987 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADNT
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        220..504
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     427..446
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             541..558
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             571..591
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      688..734
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
       61 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
      121 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
      181 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
      241 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
      301 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
      361 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
      421 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
      481 cgcaactctc tactgtttct ccatacccgt tttttgggct aacaggagga attaaccatg
      541 caccaccacc accaccaccc gtcggccggc gagaacctgt acttccaggg cgccatggac
      601 gataaggtac cggatccgaa ttcgatatcg ctagcaagct tatcgatgtc gacgtagtta
      661 actagcgtac gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      721 gcctttcgtt ttatgccatc atggccgcgg tgatcagcta gagggggcgt caggcgccgg
      781 gggcggtgtc cggcggcccc cagaggaact gcgccagttc ctccggatcg gtgaagccgg
      841 agagatccag cggggtctcc tcgaacacct cgaagtcgtg caggaaggtg aaggcgagca
      901 gttcgcgggc gaagtcctcg gtccgcttcc actgcgcccc gtcgagcagc gcggccagga
      961 tctcgcggtc gccccggaag gcgttga
//
