LOCUS       sequence-166717-        5238 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing GPR156 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GPR156_p.C763S
FEATURES             Location/Qualifiers
     source          1..5238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2638..2737)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2754..2773)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2755..2773)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2778..2794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2778..2794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3225..4016
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4109..4697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4598..4617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4851..4868
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5027..5054)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5146..5232)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggagcct gaaataaact gctctgaatt gtgtgacagt tttcctggcc
      241 aggagctgga tcggagaccc cttcatgatc tctgcaagac aacaattaca tcttcccacc
      301 acagcagtaa gaccatctct tcattatctc ctgtcctctt gggtattgtt tggacttttc
      361 tcagctgtgg acttctgctg atacttttct ttcttgcctt tacaattcac tgcaggaaga
      421 acaggattgt gaagatgtcc agtcccaatc tgaacattgt gaccttactg ggcagttgtc
      481 tcacttacag tagcgcttac ctctttggga ttcaggatgt tttagtgggg agctcaatgg
      541 aaactctcat tcagacaaga ctgtccatgc tgtgcattgg gacctccctt gtgtttggcc
      601 ccattctggg aaagagctgg cgactctaca aggtgtttac ccaaagggtc ccggacaaga
      661 gagtgattat caaagacctg cagttgctgg ggttggtggc agccctgttg atggctgatg
      721 tgatcctgct catgacgtgg gtgctgactg atcccatcca gtgcctccag attctcagtg
      781 tcagtatgac ggtgacaggg aaagacgtgt cctgcacttc gaccagcacc cacttctgtg
      841 cttcccggta ttccgatgtt tggattgctc tcatttgggg atgcaagggt ctgctcctgc
      901 tgtatggtgc ctacctggct ggcctgactg gccatgtcag ctcccctcct gtgaatcagt
      961 ccttaaccat catggtgggg gtcaacctcc ttgtactggc tgctgggctg ctttttgtag
     1021 tcaccagata cttgcattcc tggcccaacc tggtctttgg actcacatct ggagggatct
     1081 ttgtttgtac aactacaatc aactgcttca tcttcattcc ccagctgaag caatggaagg
     1141 catttgaaga ggaaaaccaa acaatcagac gcatggccaa atatttcagc actcccaaca
     1201 aaagcttcca tacccagtat ggtgaggagg agaactgcca cccgagggga gagaaaagct
     1261 ccatggagag gctcctcaca gaaaaaaatg ctgtgattga aagcctgcag gaacaagtaa
     1321 acaacgccaa agagaagatt gtgaggctga tgtcagctga gtgcacctat gacctcccag
     1381 agggggctgc cccacctgcc tcttccccga acaaggacgt ccaggcggta gcctcggtcc
     1441 acaccctggc agctgctcag gggccttcgg gtcacctctc tgactttcag aatgatcctg
     1501 gcatggctgc ccgggattcc cagtgcactt cagggccctc ctcatatgca caaagccttg
     1561 aggggcctgg gaaggactcc agcttctccc cagggaagga ggagaagata tctgactcaa
     1621 aagacttttc tgatcattta gactcaggtt gtagccagaa gccatggact gagcaaagcc
     1681 tgggtccaga aagaggagac caagtcccca tgaaccccag ccagagtctc ctaccagata
     1741 gaggcggctc agatccccag agacagaggc atctggagaa ctcagaggag cccccagagc
     1801 ggcggtcacg ggttagttca gtaatcaggg agaaacttca ggaggtctta caagatctgg
     1861 gcctgggccc tgaggcttcc ctctccaccg ccccctcttg tcatcagcaa acctggaaga
     1921 acagtgctgc cttcagcccc caaaagatgc ccctctccaa ggagctgggc tttagccctt
     1981 acatggtgag gagaaggcgg gcagctcagc gggcccgctc acactttcct ggctctgcac
     2041 cctcatctgt ggggcatcgg gcaaacagga ctgttcctgg ggcacacagc aggctacatg
     2101 tgcagaatgg ggacagcccc agcctggccc cacaaactac tgattccaga gtacgaagac
     2161 cttcttccag gaagccttca ctaccttcag atcctcaaga cagaccaggt accctggagg
     2221 gcagcaaaca aagccagaca gagcccgagg gggctagagg gagcaaagca gcctttcttc
     2281 gccagccttc tggttctggc cgggccccaa gtcctgctgc cccatgcctc tccaaagcct
     2341 cacctgactt gcctgaacag tggcagctgt ggcccccagt gccctcaggc tgtgcctccc
     2401 tgtcttctca acacagctat tttgatactg agtccagcag ctcagatgag ttcttctgcc
     2461 gctgccaccg gccctactct gaaatctgct tccagagctc ttctgactct agtgacagtg
     2521 gcacatcaga cactgaccct gagcctactg gggggctggc ttcctgggaa aagctgtggg
     2581 cccgctccaa gcctattgtg aacttcaaag atgacttgaa acccacgctg gtgtgattgc
     2641 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2701 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2761 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2821 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2881 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2941 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     3001 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3061 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3121 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3181 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     3241 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3301 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3361 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3421 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3481 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3541 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3601 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3661 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3721 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3781 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3841 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3901 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3961 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     4021 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4801 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4861 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4921 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4981 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     5041 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     5101 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     5161 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     5221 tcgttttatt tgatgcct
//
