LOCUS       sequence-166750-        4152 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing IDH2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH2_p.T331M
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2031..2130)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2147..2166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2148..2166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2171..2187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2171..2187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2618..3409
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3502..4090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3991..4010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggccggct acctgcgggt cgtgcgctcg ctctgcagag cctcaggctc
      721 gcggccggcc tgggcgccgg cggccctgac agcccccacc tcgcaagagc agccgcggcg
      781 ccactatgcc gacaaaagga tcaaggtggc gaagcccgtg gtggagatgg atggtgatga
      841 gatgacccgt attatctggc agttcatcaa ggagaagctc atcctgcccc acgtggacat
      901 ccagctaaag tattttgacc tcgggctccc aaaccgtgac cagactgatg accaggtcac
      961 cattgactct gcactggcca cccagaagta cagtgtggct gtcaagtgtg ccaccatcac
     1021 ccctgatgag gcccgtgtgg aagagttcaa gctgaagaag atgtggaaaa gtcccaatgg
     1081 aactatccgg aacatcctgg gggggactgt cttccgggag cccatcatct gcaaaaacat
     1141 cccacgccta gtccctggct ggaccaagcc catcaccatt ggcaggcacg cccatggcga
     1201 ccagtacaag gccacagact ttgtggcaga ccgggccggc actttcaaaa tggtcttcac
     1261 cccaaaagat ggcagtggtg tcaaggagtg ggaagtgtac aacttccccg caggcggcgt
     1321 gggcatgggc atgtacaaca ccgacgagtc catctcaggt tttgcgcaca gctgcttcca
     1381 gtatgccatc cagaagaaat ggccgctgta catgagcacc aagaacacca tactgaaagc
     1441 ctacgatggg cgtttcaagg acatcttcca ggagatcttt gacaagcact ataagaccga
     1501 cttcgacaag aataagatct ggtatgagca ccggctcatt gatgacatgg tggctcaggt
     1561 cctcaagtct tcgggtggct ttgtgtgggc ctgcaagaac tatgacggag atgtgcagtc
     1621 agacatcctg gcccagggct ttggctccct tggcctgatg atgtccgtcc tggtctgccc
     1681 tgatgggaag acgattgagg ctgaggccgc tcatgggacc gtcacccgcc actatcggga
     1741 gcaccagaag ggccggccca ccagcaccaa ccccatcgcc agcatctttg cctggacacg
     1801 tggcctggag caccggggga agctggatgg gaaccaagac ctcatcaggt ttgcccagat
     1861 gctggagaag gtgtgcgtgg agacggtgga gagtggagcc atgaccaagg acctggcggg
     1921 ctgcattcac ggcctcagca atgtgaagct gaacgagcac ttcctgaaca ccacggactt
     1981 cctcgacacc atcaagagca acctggacag agccctgggc aggcagtgat tgccaacttt
     2041 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2101 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2161 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2221 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2281 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2341 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     2401 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2461 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     2521 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     2581 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     2641 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     2701 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     2761 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     2821 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     2881 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     2941 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3001 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3061 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3121 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3181 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3241 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3301 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3361 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     3421 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4141 tgctcacatg tt
//
