LOCUS       sequence-166879-         492 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Adenoviral shuttle vector for XBP1u expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Zeng L, Zampetaki A, Margariti A, Pepe AE, Alam S, Martin D, Xiao Q,
            Wang W, Jin ZG, Cockerill G, Mori K, Li YS, Hu Y, Chien S, Xu Q
  TITLE     Sustained activation of XBP1 splicing leads to endothelial apoptosis
            and atherosclerosis development in response to disturbed flow.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 May 19;106(20):8326-31. doi: 
            10.1073/pnas.0903197106. Epub 2009 May 1.
  PUBMED    19416856
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903197106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-05-19- 19"; volume: "106"; issue: "20"; pages: "8326-31"
COMMENT     Sequence Label: pShuttle-Flag-XBP1u
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcatggact acaaggacga cgatgacaag gaattcagat ctggtaccct
      121 ggtggtggtg gcagccgcgc cgaacccggc cgacgggacc cctaaagttc tgcttctgtc
      181 ggggcagccc gcctccgccg ccggagcccc ggccggccag gccctgccgc tcatggtgcc
      241 agcccagaga ggggccagcc cggaggcagc gagcgggggg ctgccccagg cgcgcaagcg
      301 acagcgcctc acgcacctga gccccgagga gaaggcgctg aggaggaaac tgaaaaacag
      361 agtagcagct cagactgcca gagatcgaaa gaaggctcga atgagtgagc tggaacagca
      421 agtggtagat ttagaagaag agaaccaaaa acttttgcta gaaaatcagc ttttacgaga
      481 gaaaactcat gg
//
