LOCUS       sequence-167065-         826 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  rAAV-based template for genome engineering of protein C-termini 
            containing PQS1 and 3xFLAG tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-MCS-PQS1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     291..310
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(453..470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    459..683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(708..741)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
       61 tactcggatg gaagccggtc ttgtcaatca ggatgatctg gacgaagagc atcaggggct
      121 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      181 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      241 attcatcgac tgtggccggc tgggtgtggc ggatcgctat caggacatag cgttggctac
      301 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      361 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      421 aggggatcaa ttctctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
      481 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
      541 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
      601 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
      661 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctata acttcgtata
      721 gcatacatta tacgaagtta tagtaatcga taactcgaga aggatccaag gtaccaagct
      781 agcaaaagcg gccgcaggaa cccctagtga tggagttggc cactcc
//
