LOCUS       sequence-167091-        5334 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Donor vector containing STAT6 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAT6_p.N417Y
FEATURES             Location/Qualifiers
     source          1..5334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2734..2833)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2850..2869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2851..2869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2874..2890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2874..2890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3321..4112
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4205..4793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4694..4713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4947..4964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5123..5150)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5242..5328)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtctctg tggggtctgg tctccaagat gcccccagaa aaagtgcagc
      241 ggctctatgt cgactttccc caacacctgc ggcatcttct gggtgactgg ctggagagcc
      301 agccctggga gttcctggtc ggctccgacg ccttctgctg caacttggct agtgccctac
      361 tttcagacac tgtccagcac cttcaggcct cggtgggaga gcagggggag gggagcacca
      421 tcttgcaaca catcagcacc cttgagagca tatatcagag ggaccccctg aagctggtgg
      481 ccactttcag acaaatactt caaggagaga aaaaagctgt tatggaacag ttccgccact
      541 tgccaatgcc tttccactgg aagcaggaag aactcaagtt taagacaggc ttgcggaggc
      601 tgcagcaccg agtaggggag atccaccttc tccgagaagc cctgcagaag ggggctgagg
      661 ctggccaagt gtctctgcac agcttgatag aaactcctgc taatgggact gggccaagtg
      721 aggccctggc catgctactg caggagacca ctggagagct agaggcagcc aaagccctag
      781 tgctgaagag gatccagatt tggaaacggc agcagcagct ggcagggaat ggcgcaccgt
      841 ttgaggagag cctggcccca ctccaggaga ggtgtgaaag cctggtggac atttattccc
      901 agctacagca ggaggtaggg gcggctggtg gggagcttga gcccaagacc cgggcatcgc
      961 tgactggccg gctggatgaa gtcctgagaa ccctcgtcac cagttgcttc ctggtggaga
     1021 agcagccccc ccaggtactg aagactcaga ccaagttcca ggctggagtt cgattcctgt
     1081 tgggcttgag gttcctgggg gccccagcca agcctccgct ggtcagggcc gacatggtga
     1141 cagagaagca ggcgcgggag ctgagtgtgc ctcagggtcc tggggctgga gcagaaagca
     1201 ctggagaaat catcaacaac actgtgccct tggagaacag cattcctggg aactgctgct
     1261 ctgccctgtt caagaacctg cttctcaaga agatcaagcg gtgtgagcgg aagggcactg
     1321 agtctgtcac agaggagaag tgcgctgtgc tcttctctgc cagcttcaca cttggccccg
     1381 gcaaactccc catccagctc caggccctgt ctctgcccct ggtggtcatc gtccatggct
     1441 accaagacaa caatgccaaa gccactatcc tgtgggacaa tgccttctct gagatggacc
     1501 gcgtgccctt tgtggtggct gagcgggtgc cctgggagaa gatgtgtgaa actctgaacc
     1561 tgaagttcat ggctgaggtg gggaccaacc gggggctgct cccagagcac ttcctcttcc
     1621 tggcccagaa gatcttcaat gacaacagcc tcagtatgga ggccttccag caccgttctg
     1681 tgtcctggtc gcagttcaac aaggagatcc tgctgggccg tggcttcacc ttttggcagt
     1741 ggtttgatgg tgtcctggac ctcaccaaac gctgtctccg gagctactgg tctgaccggc
     1801 tgatcattgg cttcatcagc aaacagtacg ttactagcct tcttctcaat gagcccgacg
     1861 gaacctttct cctccgcttc agcgactcag agattggggg catcaccatt gcccatgtca
     1921 tccggggcca ggatggctct ccacagatag agaacatcca gccattctct gccaaagacc
     1981 tgtccattcg ctcactgggg gaccgaatcc gggatcttgc tcagctcaaa aatctctatc
     2041 ccaagaagcc caaggatgag gctttccgga gccactacaa gcctgaacag atgggtaagg
     2101 atggcagggg ttatgtccca gctaccatca agatgaccgt ggaaagggac caaccacttc
     2161 ctaccccaga gctccagatg cctaccatgg tgccttctta tgaccttgga atggcccctg
     2221 attcctccat gagcatgcag cttggcccag atatggtgcc ccaggtgtac ccaccacact
     2281 ctcactccat ccccccgtat caaggcctct ccccagaaga atcagtcaac gtgttgtcag
     2341 ccttccagga gcctcacctg cagatgcccc ccagcctggg ccagatgagc ctgccctttg
     2401 accagcctca cccccagggc ctgctgccgt gccagcctca ggagcatgct gtgtccagcc
     2461 ctgaccccct gctctgctca gatgtgacca tggtggaaga cagctgcctg agccagccag
     2521 tgacagcgtt tcctcagggc acttggattg gtgaagacat attccctcct ctgctgcctc
     2581 ccactgaaca ggacctcact aagcttctcc tggaggggca aggggagtcg gggggagggt
     2641 ccttgggggc acagcccctc ctgcagccct cccactatgg gcaatctggg atctcaatgt
     2701 cccacatgga cctaagggcc aaccccagtt ggtgcccaac tttcttgtac aaagttggca
     2761 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2821 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2881 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2941 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     3001 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     3061 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     3121 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     3181 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     3241 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     3301 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     3361 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3421 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3481 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3541 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3601 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3661 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3721 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3781 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3841 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3901 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3961 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     4021 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     4081 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     4141 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4321 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4561 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5041 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     5101 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     5161 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     5221 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     5281 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct
//
