LOCUS       sequence-167173-         992 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-877-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    145..279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(182..201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(589..610)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     938..957
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaaattttat cgatcacgag actagcctcg agagatctga tcataatcag ccataccaca
       61 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      181 ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt
      241 ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg
      301 cctgtcatcg aattcctgca gcccggtgca tgactaagct agtaccggtg tagaggagat
      361 ggcgcagggg tagaggagat ggcgcagggg tagaggagat ggcgcagggg tagaggagat
      421 ggcgcagggg tagaggagat ggcgcagggg tagaggagat ggcgcagggg ctacgacgat
      481 tggtactccc cagttaaggt cggctagctc agttagcctc ccccatctct cgacgcggcc
      541 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      601 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      661 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      721 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      781 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      841 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      901 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      961 gaagaagatg gtgcgctcct ggacgtagcc tt
//
