LOCUS       sequence-167199-         900 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  1st Pro5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pHPro51
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    323..344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(359..364,365..382,383..389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    397..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     402..424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     421..437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     421..437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             433..690
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNWSREVAVPLHLHRETDPLESTCRHASLALAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     primer_bind     complement(534..551)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(534..550)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(543..565)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(705..722)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
       61 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt
      121 taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag
      181 cggatgaatg gcagaaattc agcttggccc agtgccaagc tccaatacgc aaaccgcctc
      241 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      301 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      361 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      421 caggaaacag ctatgaccat gattacgaat tggtctcggg aggtggcggt tcccctccac
      481 ctccaccgcg agaccgatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
      541 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      601 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      661 caacagttgc gcagcctgaa tggcgaatga gcttcttccg cttcctcgct cactgactcg
      721 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
//
